IDENTIFICATION OF VIRAL PATHOGENS BY SEROLOGICAL TECHNIQUES AND MOLECULAR DIAGNOSTIC TECHNIQUES IN APRICOT, PEACH AND ALMOND

Q4 Agricultural and Biological Sciences

Fruit Growing Research Pub Date : 2023-12-12 DOI:10.33045/fgr.v39.2023.17

C. Plopa, Adina Floricica Iancu, C. Gavăț, G. Lămureanu

引用次数: 0

Abstract

In order to obtain plants of the biological category Pre-basic, plants of the genotypes ꞌOlimpꞌ and ꞌDe Valuꞌ in apricot, ꞌRalucaꞌ, ꞌMonicaꞌ, ꞌTomis 1ꞌ in peach, ꞌMirelaꞌ and ꞌVeronicaꞌ in almond were evaluated for the presence of virus-type harmful organisms. Diagnosis was made by the serological DAS-ELISA method for ACLSV, ApMV, PPV, PDV, PNRSV and SLRSV and by the molecular RT-PCR test for ESFY, ApLV and PLMVd. No positive samples were found in apricot. In peach the viruses were identified in ꞌMonicaꞌ: ApLV in 57.15% of the plants tested, PNRSV in 14.29% and PLMVd in 7.15% of the plants. Two of the positive plants had mixed infections produced by PNRSV +ApLV and PNRSV +PLMVd. In peach genotype ꞌTomis 1ꞌ, 23.08 % plants were found infected with ApMV, 7.7 % with ACLSV and 7.7 % with PPV. ACLSV and PPV infections were identified in the same plant. In almond the diagnosis revealed a mixed infection with ACLSV + ApMV (7.7 % of plants from ꞌMirelaꞌ variety).

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通过血清学技术和分子诊断技术鉴定杏、桃和杏仁中的病毒病原体

桃中的ꞌMonicaꞌ、ꞌTomis 1ꞌ，杏中的ꞌMirelaꞌ和ꞌVeronicaꞌ进行了评估，以确定是否存在病毒型有害生物。对 ACLSV、ApMV、PPV、PDV、PNRSV 和 SLRSV 采用血清 DAS-ELISA 法进行诊断，对 ESFY、ApLV 和 PLMVd 采用分子 RT-PCR 检测法进行诊断。杏中没有发现阳性样本。在桃ꞌMonicaꞌ中发现了病毒：57.15%的受测植株感染了 ApLV，14.29%的植株感染了 PNRSV，7.15%的植株感染了 PLMVd。其中两株阳性植株受到 PNRSV +ApLV 和 PNRSV +PLMVd 的混合感染。在桃基因型ꞌTomis 1ꞌ中，23.08%的植株感染了 ApMV，7.7%感染了 ACLSV，7.7%感染了 PPV。在同一株植物上发现了 ACLSV 和 PPV 感染。在杏仁中，诊断发现 ACLSV + ApMV 混合感染（ꞌMirelaꞌ 品种的 7.7 % 的植株）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fruit Growing Research Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

0.10

自引率

0.00%

发文量

审稿时长

8 weeks

期刊介绍： FRUIT GROWING RESEARCH is an international journal, which has been issued under the care of the Research Institute for Fruit Growing Pitesti, Romania. Fruit growing research is an annual journal aimed at disseminating significant research and original papers on genetic resources, breeding of deciduous fruit trees species; fruit sustainable technologies – environment protection; propagation, virology and tissue culture, postharvest, that can be of interest to a wide audience of plant scientists in all areas of fruit growing. An international Editorial Board advises the journal.