RNA Sequencing Analysis of Changes in Pancreatic RNA in Tree Shrews with Traumatic Systemic Inflammatory Response Syndrome after Treatment with Umbilical Cord Mesenchymal Stem Cells

Abstract

Background: Systemic inflammatory response syndrome (SIRS) is a systemic nonspecific inflammatory response to severe injury caused by infectious or noninfectious factors. Methods: The impact method was used to make unilateral femoral comminuted fractures in tree shrews and lipopolysaccharide was injected intravenously to create a traumatic systemic inflammatory response syndrome model. The treatment group was infused with tree shrew umbilical cord mesenchymal stem cells via the tail vein. Result: After 10 days of treatment, 3 tree shrews were killed in each of the control group, the model group and the treatment group and pancreatic tissue was taken for RNA sequencing analysis. The results showed statistically significant changes in some genes. The two genes with the most significantly changed expression levels were ENSTBEG00000013408 (gene name TMEM211) and ENSTBEG00000006837 (gene name C1QL3). Their expression levels were increased in the model group and decreased after treatment. The changes in inflammatory factors indicate that the model group has an obvious inflammatory response and the treatment of umbilical cord mesenchymal stem cells has the effect of reducing inflammatory factors, which further proves the anti-inflammatory effect of umbilical cord mesenchymal stem cells. There was also an obvious change in the expression of the ENSTBEG00000021330 gene (gene name ATP8), which decreased in the model group and increased after treatment, indicating that ATP8 is a gene that contributes to the recovery of systemic inflammatory response syndrome. In the future, we may use this gene to treat systemic inflammatory response syndrome.