Antifungal Activities of Erythrina senegalensis Leaves Partitioned Extracts on the Germs Responsible for Opportunistic Cryptococcosis of HIV/AIDS

Abstract

Introduction: The mortality rate remains very high (45.1%) among HIV-infected subjects with cryptococcosis in developing countries due to the inaccessibility and toxicity of conventional antifungals. Thus, the alternative search for new, more effective and less expensive antifungal molecules required this study on Erythrina Senegalensis, a plant used against mycoses in ivorian traditional medicine. Methodology: The double dilution method in liquid medium and the diffusion method in solid medium were used for the antifungal tests of partitions of the of Erythrina senegalensis leaves hydroalcoholic extract, in comparison with conventional antifungals. Results: Among the partitions of Erythrina senegalensis leaves hydroalcoholic extract obtained and tested, the partition with hexane was the most active with an MIC of 3.12 mg/ml, an IC50 of 1.55 mg/ml. Having obtained an inhibition diameter of 15 mm, this hexane partition containing flavonoids, sterols and terpenes has antifungal activity on Cryptococcus neoformans in the same way as amphotericin B and Fluconazole. Conclusion: Thanks to its fungistatic action, the Erythrina senegalensis leaves hexane partition has a fungistatic action on HIV/AIDS opportunistic cryptococcosis.