Study of the protective effect of ginseng against testicular oxidative stress biomarkers and its gene expression induced by ciprofloxacin

Bionatura Pub Date : 2023-12-15 DOI:10.21931/rb/2023.08.04.20

Haitham M. Mokhimar, H. Elsawah, Mohamed M. Kandiel, Faten E. Elsaid, A. El-Mahmoudy

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Abstract

Ciprofloxacin is the first-choice member of the fluoroquinolone antibacterials for treating testicular infections, but it may harm testicular tissue because of oxidative stress. Many mechanisms are involved, like decreasing antioxidant enzymes and suppressing gene expression. This study intends to investigate the possible protective role of ginseng against ciprofloxacin-induced testicular oxidative stress and its mechanism, if any. For this purpose, 50 adult male albino rats were randomly divided into five groups, ten rats in each group. Rats in group 1 received only ciprofloxacin at a daily dose of 156.46 mg/kg. Rats in groups 2, 3 and 4 received ciprofloxacin in a daily dose of 156.46 mg/kg, ginseng in two doses of 100 and 200 mg/kg, and vitamin E as a standard in a daily dose of 100 mg/kg, respectively. Group 5 served as control and received carboxymethylcellulose in normal saline. All these treatments were applied orally during 14 14-day experimental courses. Half the animals in each group were euthanized on day 15 from the start of the treatment, while the second half was euthanized on day 60. Both testes were dissected, immediately frozen, and evaluated for oxidative stress biomarkers and gene expression antioxidant enzymes. We found that ciprofloxacin significantly (P ≤ 0.05) increased MDA and decreased total antioxidant capacity (TAC), superoxide dismutase (SOD) and catalase (CAT) compared to the control group. Also, the drug downregulated gene expression of SOD and CAT. Compared to all groups, the co-administration of ginseng or vitamin E with ciprofloxacin almost normalized antioxidant enzymes and upregulated the tested gene expressions. It could be concluded that ginseng ameliorates the testicular adverse effect of ciprofloxacin. So, it is highly recommended to be used as an adjunct remedy during ciprofloxacin administration for its antioxidant properties. Keywords: Ciprofloxacin, Gene expression, Ginseng, Infertility, ROS, Testicular oxidative stress, Vitamin-E.

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研究人参对环丙沙星诱导的睾丸氧化应激生物标志物及其基因表达的保护作用

环丙沙星是氟喹诺酮类抗菌药物中治疗睾丸感染的首选药物，但它可能会因氧化应激而损害睾丸组织。其中涉及多种机制，如减少抗氧化酶和抑制基因表达。本研究旨在探讨人参对环丙沙星引起的睾丸氧化应激的可能保护作用及其机制。为此，研究人员将 50 只成年雄性白化大鼠随机分为 5 组，每组 10 只。第 1 组大鼠只服用环丙沙星，每日剂量为 156.46 毫克/千克。第 2、3 和 4 组大鼠分别接受每日剂量为 156.46 毫克/千克的环丙沙星、100 毫克/千克和 200 毫克/千克两种剂量的人参以及每日剂量为 100 毫克/千克的标准维生素 E。第 5 组为对照组，在生理盐水中加入羧甲基纤维素。所有这些治疗均在 14 个为期 14 天的实验疗程中口服。每组一半动物在治疗开始后第 15 天安乐死，另一半动物在第 60 天安乐死。解剖并立即冷冻两组动物的睾丸，并对氧化应激生物标志物和抗氧化酶的基因表达进行评估。我们发现，与对照组相比，环丙沙星能显著增加MDA（P≤0.05），降低总抗氧化能力（TAC）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）。此外，药物还下调了 SOD 和 CAT 的基因表达。与所有组别相比，人参或维生素 E 与环丙沙星联合用药几乎使抗氧化酶恢复正常，并上调了测试基因的表达。由此可以得出结论，人参能改善环丙沙星对睾丸的不良影响。因此，强烈建议在环丙沙星用药期间使用人参作为辅助疗法，因为人参具有抗氧化特性。关键词：环丙沙星环丙沙星基因表达人参不育症 ROS 睾丸氧化应激维生素E

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