Improving Foodborne Pathogen Control Using Green Nanosized Emulsions of Plectranthus hadiensis Phytochemicals

IF 2.5 Q3 CHEMISTRY, PHYSICAL
Lucía Carolina Vega-Hernández, J. C. Serrano-Niño, C. Velázquez-Carriles, Alma H. Martínez-Preciado, Adriana Cavazos-Garduño, J. Silva-Jara
{"title":"Improving Foodborne Pathogen Control Using Green Nanosized Emulsions of Plectranthus hadiensis Phytochemicals","authors":"Lucía Carolina Vega-Hernández, J. C. Serrano-Niño, C. Velázquez-Carriles, Alma H. Martínez-Preciado, Adriana Cavazos-Garduño, J. Silva-Jara","doi":"10.3390/colloids8010003","DOIUrl":null,"url":null,"abstract":"Every year, millions of foodborne illnesses with thousands of deaths occur worldwide, which is why controlling foodborne pathogens is sought. In this study, nanoemulsions of phytochemicals extracted from Plectranthus hadiensis var. tomentosus (PHT) were obtained, and their antioxidant and antimicrobial capacities were evaluated. PHT extracts were obtained by maceration, ultrasound, and Naviglio methods, and their antimicrobial activity against Staphylococcus aureus, Listeria monocytogenes, Escherichia coli, and Salmonella enterica was determined by the microdilution method. The extract with the highest antimicrobial activity was obtained by Naviglio with a minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 12.5 and 25 mg/mL, respectively, for all bacterial strains. The nanoemulsion (o/w) made with Tween 40, 5% extract, and 50% ultrasonic amplitude had a globule size of 4.4 nm, a polydispersity index of 0.48, and a surface charge of −0.08 mV and remained stable for 30 days. This nanosystem presented significantly higher antimicrobial and antioxidant activity than the free extract. Thus, the nanoencapsulation of the phytochemicals in the PHT extracts is an alternative to protect and enhance their biological activity against pathogenic microorganisms.","PeriodicalId":10433,"journal":{"name":"Colloids and Interfaces","volume":"3 2","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Colloids and Interfaces","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/colloids8010003","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
引用次数: 0

Abstract

Every year, millions of foodborne illnesses with thousands of deaths occur worldwide, which is why controlling foodborne pathogens is sought. In this study, nanoemulsions of phytochemicals extracted from Plectranthus hadiensis var. tomentosus (PHT) were obtained, and their antioxidant and antimicrobial capacities were evaluated. PHT extracts were obtained by maceration, ultrasound, and Naviglio methods, and their antimicrobial activity against Staphylococcus aureus, Listeria monocytogenes, Escherichia coli, and Salmonella enterica was determined by the microdilution method. The extract with the highest antimicrobial activity was obtained by Naviglio with a minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 12.5 and 25 mg/mL, respectively, for all bacterial strains. The nanoemulsion (o/w) made with Tween 40, 5% extract, and 50% ultrasonic amplitude had a globule size of 4.4 nm, a polydispersity index of 0.48, and a surface charge of −0.08 mV and remained stable for 30 days. This nanosystem presented significantly higher antimicrobial and antioxidant activity than the free extract. Thus, the nanoencapsulation of the phytochemicals in the PHT extracts is an alternative to protect and enhance their biological activity against pathogenic microorganisms.
利用绿色纳米级海带植物化学乳剂改善食源性病原体控制
全世界每年都会发生数百万起食源性疾病,造成数千人死亡,这就是人们寻求控制食源性病原体的原因。本研究从 Plectranthus hadiensis var. tomentosus(PHT)中提取了植物化学物质的纳米乳液,并对其抗氧化和抗菌能力进行了评估。PHT 提取物是通过浸渍法、超声波法和纳维格利奥法提取的,它们对金黄色葡萄球菌、李斯特菌、大肠杆菌和肠炎沙门氏菌的抗菌活性是通过微量稀释法测定的。Naviglio 提取物的抗菌活性最高,对所有细菌菌株的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)分别为 12.5 毫克/毫升和 25 毫克/毫升。用吐温 40、5% 的提取物和 50%的超声波振幅制成的纳米乳液(o/w)的球粒大小为 4.4 nm,多分散指数为 0.48,表面电荷为 -0.08 mV,并且在 30 天内保持稳定。这种纳米系统的抗菌和抗氧化活性明显高于游离提取物。因此,对 PHT 提取物中的植物化学物质进行纳米封装是保护和提高其抗病原微生物生物活性的一种替代方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Colloids and Interfaces
Colloids and Interfaces CHEMISTRY, PHYSICAL-
CiteScore
3.90
自引率
4.20%
发文量
64
审稿时长
10 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信