Biogenic Synthesis of Gold Nanoparticles using Bark Extract of Bauhinia variegata: Antibacterial and in vitro Anticancer study

Abstract

Our study revealed the biological synthesis of gold nanoparticles (AuNPs) using aqueous bark extract of plant named Bauhinia variegata (Kaanchnar). Biomolecules present in the plants act as a reducing and capping agent. In this reduction reaction, gold (III) metal was reduced by biomolecules present in plants leading to formation of AuNPs. Biosynthesized AuNPs were characterized by spectral analysis (XRD, FTIR, UV-visible) and imaging microscopy. Antibacterial study of the biosynthesized AuNPs was examined against Gram-positive (Bacillus subtilis) and Gram-negative (Escherichia coli) bacteria using agar well diffusion method and AuNPs exhibited an excellent inhibition zone against mentioned bacteria. In vitro anticancer study of AuNPs was performed on MCF-7 cell line. Compared to standard drug, AuNPs exhibited potent anticancer activity with the IC50 value 15.55 μg/ml.