Screening of Legionella pneumophila from Well Water in Magetan Regency, Indonesia

Indonesian Journal of Tropical and Infectious Disease Pub Date : 2023-12-07 DOI:10.20473/ijtid.v11i3.48755

Nafisah Nurul Hapsari, Muhammad Ridho, Hafid Kurniawan, T. H. Sucipto, Nur Syamsiatul Fajar, Eduardus Bimo, Aksono Herupradopo, S. Fauziyah, Alicia Margaretta Widya, Febriana Aquaresta

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Abstract

Legionellosis is a respiratory infection caused by Legionella pneumophila, a bacterium that can infect protozoa and human lung cells. The disease can be mild or severe, and sometimes fatal, especially in immunocompromised people. Some types of antibiotics that can be used to treat Legionella disease are macrolides such as rifampicin, azithromycin and clarithromycin, as well as fluoroquinolones such as levofloxacin and moxifloxacin. The aim was to investigate the distribution and prevalence of Legionella in well water. This study collected and tested water samples from a different well water in Magetan Regency, East Java, Indonesia using DNA extraction, two-step PCR methods, and visualization with 1.5% agarose gel in UV transluminator. The results were visualized with QGIS 3.28.6 and compared with other tests. The results showed that none of the eight water samples were contaminated with L. pneumophila compared with positive control (403 bp).

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印度尼西亚马格丹县井水中嗜肺军团菌的筛查

军团菌病是一种由嗜肺军团菌引起的呼吸道感染，这种细菌可感染原生动物和人类肺细胞。这种疾病可轻可重，有时甚至致命，尤其是在免疫力低下的人群中。可用于治疗军团菌病的抗生素有大环内酯类，如利福平、阿奇霉素和克拉霉素，以及氟喹诺酮类，如左氧氟沙星和莫西沙星。目的是调查井水中军团菌的分布和流行情况。本研究收集并检测了印度尼西亚东爪哇马格丹县不同井水中的水样，采用了 DNA 提取、两步 PCR 方法，并在紫外转光器中用 1.5% 琼脂糖凝胶显像。结果用 QGIS 3.28.6 进行了可视化，并与其他测试结果进行了比较。结果表明，与阳性对照（403 bp）相比，8 份水样均未受到嗜肺菌污染。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Indonesian Journal of Tropical and Infectious Disease

自引率

0.00%

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审稿时长

12 weeks