Haematological Profile of Various Inbred Strains of Mice Maintained at IMTech Centre for Animal Resources and Experimentation (iCARE)

Abstract

Animal models are frequently employed in scientific studies to collect data from an entire set of experiments. Haematological tests on these laboratory mice are essential for confirming scientific findings because diverse mouse strains have been used to examine various diseases and novel therapeutic methods. This work is aimed to establish haematological values in mouse strains used in biomedical research. Blood samples were taken from the retro-orbital sinus of mice from various strains and haematological parameters including total White Blood Cells (WBC), Red Blood Cells (RBC), Haemoglobin (HGB), Haematocrit (HCT), Mean Corpuscular Volume (MCV), Mean Corpuscular Haemoglobin (MCH), Mean Corpuscular Haemoglobin Concentration (MCHC), Red Blood Cell Dimension Width ( RDW), platelets, Mean Platelet Volume (MPV) and Platelet Dimension Width (PDW) were assessed. Compared to other strains (BALB/c, M:9.61, F:9.51; C57BL6/N, M:10.13, F:10.73; DBA/2, M:11.96, F:10.88), the mean values of WBCs (*103/μL) in DBA/1 mice were higher (M:14.02; F:14.65) and lower in NOD (M:7.14; F:6.54). The mean RBCs in different strains of mice used in this study ranged from 8.78-11.67*106/μL. Male mean haemoglobin valves in BALB/c, C57BL/6N, DBA/1, DBA/2 and NOD were 14.60, 13.32, 13.00, 14.35 and 13.48 (g/dL), respectively, while in females were 14.3, 14.09, 13.11, 12.65 and 13.24 (g/dL), respectively. The mean platelet counts in NOD mice (M:1826.60 and F:1557.80*103/μL) were significantly higher than other strains. Haematological profiles of the mice used in the study were consistent as reported previously for mice maintained in different animal facilities. The haematological values presented in this study can be used as reference values in biomedical research.