Nicotine is a potent extracellular polysaccharide inducer in Fusobacterium nucleatum biofilms

Q4 Dentistry

Brazilian Journal of Oral Sciences Pub Date : 2023-12-12 DOI:10.20396/bjos.v22i00.8671137

A. Matos, V. Barão, Richard Lee Gregory

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引用次数: 0

Abstract

The purpose of this in vitro study was to analyze the influence of nicotine on the extracellular polysaccharides in Fusobacterium nucleatum biofilm. Methods: F. nucleatum (ATCC 10953) biofilms supplemented with different concentrations of nicotine (0, 0.5, 1, 2, 4, and 8 mg/mL) were grown in two different BHI broth conditions [no sucrose and 1% sucrose]. Extracellular polysaccharides assay, pH measurements, and a spectrophotometric assay were performed. Data were submitted for ANOVA and Tukey honestly significant difference analyses (HSD) tests (α =.05). Results: Extracellular polysaccharides synthesis was influenced by an interaction between nicotine concentrations and growth medium solution containing sucrose (P<.05). The pH values declined in the sucrose-exposed biofilm were greater than in the group exposed only to nicotine (P<.05). The biofilm exposed to sucrose and nicotine had a higher total biofilm growth (P<.05) than the nicotine-treated biofilm without sucrose. Conclusions: Regardless of sucrose exposure, biofilms exposed to different nicotine concentrations influenced the amount of extracellular polysaccharides.

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尼古丁是核酸镰刀菌生物膜中一种有效的胞外多糖诱导剂

本体外研究旨在分析尼古丁对核分枝杆菌生物膜胞外多糖的影响。研究方法将添加了不同浓度尼古丁（0、0.5、1、2、4 和 8 mg/mL）的 F. nucleatum（ATCC 10953）生物膜置于两种不同的 BHI 肉汤条件（无蔗糖和 1%蔗糖）中培养。进行了胞外多糖测定、pH 值测量和分光光度测定。数据经方差分析和 Tukey 诚实显著差异分析（HSD）检验（α =.05）。结果细胞外多糖的合成受尼古丁浓度与含蔗糖的生长培养基溶液之间相互作用的影响（P<.05）。暴露于蔗糖的生物膜的 pH 值下降幅度大于仅暴露于尼古丁的生物膜组（P<.05）。接触蔗糖和尼古丁的生物膜比未接触蔗糖的尼古丁处理生物膜的生物膜总生长量更高（P<.05）。结论：无论是否接触蔗糖，接触不同浓度尼古丁的生物膜都会影响细胞外多糖的数量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Brazilian Journal of Oral Sciences Dentistry-Dentistry (all)

CiteScore

0.60

自引率

0.00%

发文量

审稿时长

20 weeks

期刊介绍： The Brazilian Journal of Oral Sciences is an international non-profit journal, which publishes full-Length papers, original research reports, literature reviews, special reports, clinical cases, current topics and short communications, dealing with dentistry or related disciplines.