Effect of Osmolytes on Photoassembly of Functionally Active Mn4CaO5 Cluster in Mn-Depleted Photosystem II Preparations Isolated from Spinach Leaves

IF 3.1 3区农林科学 Q1 HORTICULTURE

Horticulturae Pub Date : 2023-12-14 DOI:10.3390/horticulturae9121339

D. Yanykin, Dina V. Kazantseva, A. Khorobrykh

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Abstract

The effect of osmolytes (trehalose, sucrose, betaine, D-glucose and hydroxyectoine) on the photoassembly of the functionally active inorganic core of the water-oxidizing complex (Mn4CaO5 cluster) in Mn-depleted PSII preparations (apo-WOC-PSII) was investigated. It was revealed that the efficiency of the photoassembly of the Mn4CaO5 cluster was considerably (three times) increased in the presence of 1 M disaccharides (trehalose or sucrose) in contrast to other osmolytes. It was found that the osmolytes (especially trehalose or sucrose) improved the redox interaction of exogenous Mn2+ with apo-WOC-PSII, enhanced the protective effect of Mn2+ against the photoinhibition of apo-WOC-PSII, protected CaCl2-treated PSII preparations against thermoinactivation, and stabilized the water-oxidizing complex and electron transport from QA to QB in native PSII preparations during heat treatment. It is suggested that the ability of osmolytes to enhance the efficiency of the photoassembly of a Mn4CaO5 cluster depends on their effect on the following key processes: the redox interaction of Mn2+ with apo-WOC-PSII; the stability of apo-WOC-PSII to photoinhibition during the photoactivation procedure; and the stabilization of both the newly assembled functionally active Mn4CaO5 cluster and the electron transfer from QA to QB.

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溶液对从菠菜叶中分离出来的缺锰光系统 II 制剂中功能活跃的 Mn4CaO5 簇的光组装的影响

研究了渗透溶质（树胶糖、蔗糖、甜菜碱、D-葡萄糖和羟基乙二胺）对缺锰 PSII 制剂（apo-WOC-PSII）中水氧化复合物（Mn4CaO5 团簇）功能活性无机核心的光组装的影响。研究发现，与其他渗透剂相比，在 1 M 二糖（树海糖或蔗糖）存在下，Mn4CaO5 团簇的光组装效率大大提高（三倍）。研究发现，渗透溶质（尤其是树胶糖或蔗糖）改善了外源 Mn2+ 与 apo-WOC-PSII 的氧化还原相互作用，增强了 Mn2+ 对 apo-WOC-PSII 光抑制的保护作用，保护 CaCl2 处理的 PSII 制剂免受热失活，并稳定了原生 PSII 制剂在热处理过程中的水氧化复合物和从 QA 到 QB 的电子传递。研究表明，渗透剂提高 Mn4CaO5 团簇光组装效率的能力取决于它们对以下关键过程的影响：Mn2+ 与 apo-WOC-PSII 的氧化还原相互作用；apo-WOC-PSII 在光激活过程中对光抑制的稳定性；以及新组装的具有功能活性的 Mn4CaO5 团簇和从 QA 到 QB 的电子传递的稳定性。

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来源期刊

Horticulturae HORTICULTURE-

CiteScore

3.50

自引率

19.40%

发文量

998

期刊介绍：