Molecular identification of bovine gammaherpesvirus 6 in water buffaloes (Bubalus bubalis) with histological evidence of pulmonary and intestinal lesions

Mariana Motta de Castro, A. M. Dall Agnol, Vanessa Resende Rocha Tavares, A. A. C. Xavier, Flavia Helena Pereira Silva, Julia Raisa Ximenes Figueiredo, Rafaelli Ferreira Almeida, A. Alfieri, S. A. Headley
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Abstract

Bovine gammaherpesvirus 6 (BoGHV6), formerly known as bovine lymphotropic virus, belongs to the genus Macavirus, which includes the porcine lymphotropic herpesviruses (PLHV) and members collectively referred to as malignant catarrhal fever virus (MVFV) group. This study investigated the possible participation of BoGHV6 in the development of intestinal and pulmonary diseases of buffaloes. Intestinal and pulmonary fragments were obtained from 19 buffaloes from Goiás, Central-western Brazil. Histopathology revealed interstitial pneumonia in all pulmonary fragments evaluated, while two of these had suppurative bronchopneumonia. Furthermore, all intestinal fragments demonstrated atrophic enteritis. BoGHV6 DNA was amplified from 31.6% (6/19) of the buffaloes with interstitial pneumonia and in 26.3% (5/19) with atrophic enteritis. The phylogenetic evaluation revealed that the strain of BoGHV6 amplified from these buffaloes had 100% nucleotide (nt) sequence identity with the reference strains of BoGHV6, but only 69.5 to 73.7% and 65.8 – 69.5% nt sequence identity with members of the MCFV and the PLHV, respectively. Additionally, molecular assays to detect important pathogens of ruminants did not amplify the respective nucleic acids in the tissues evaluated.  These results demonstrated that BoGHV6 was amplified from tissues of buffaloes with histopathologic diagnoses of interstitial pneumonia and atrophic enteritis, suggesting the possible participation of this virus in the development of the intestinal and pulmonary lesions herein identified.
在水牛(Bubalus bubalis)中进行牛伽马疱疹病毒 6 的分子鉴定,并提供肺部和肠道病变的组织学证据
牛γ疱疹病毒6(BoGHV6)以前被称为牛淋巴细胞病毒,属于巨细胞病毒属,其中包括猪淋巴细胞疱疹病毒(PLHV)和统称为恶性卡他热病毒(MVFV)组的成员。本研究调查了BoGHV6可能参与水牛肠道和肺部疾病的发病过程。从巴西中西部戈亚斯州的 19 头水牛身上获取了肠道和肺部碎片。组织病理学结果显示,所有被评估的肺部碎片均患有间质性肺炎,其中两块碎片还患有化脓性支气管肺炎。此外,所有肠道片段均表现为萎缩性肠炎。在31.6%(6/19)的间质性肺炎水牛和26.3%(5/19)的萎缩性肠炎水牛体内分别扩增出了BoGHV6 DNA。系统进化评估显示,从这些水牛中扩增出的BoGHV6菌株与BoGHV6参考菌株的核苷酸(nt)序列同一性为100%,但与MCFV和PLHV成员的nt序列同一性分别仅为69.5%至73.7%和65.8%至69.5%。此外,检测反刍动物重要病原体的分子检测方法也没有在所评估的组织中扩增出相应的核酸。 这些结果表明,在组织病理学诊断为间质性肺炎和萎缩性肠炎的水牛组织中扩增出了BoGHV6,表明这种病毒可能参与了肠道和肺部病变的发生。
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