Increased Yields of the Guanine Oxidative Damage Product Imidazolone Following Exposure to LED Light

Reactions Pub Date : 2023-12-16 DOI:10.3390/reactions4040046

Taishu Kawada, Moka Maehara, Katsuhito Kino

引用次数: 0

Abstract

Among the bases of DNA, guanine is the most easily oxidized. Imidazolone (Iz) is a guanine oxidative damage, and we sought to generate Iz-containing oligomers. In this paper, we describe the methods and conditions to increase the yield of Iz by employing photooxidation reactions using light-emitting diodes (LEDs) with emission wavelengths of 365 nm and 450 nm. For photooxidation performed with the 450 nm LED source at light intensities of 2.75–275 mW/cm2, peak yields of Iz were 35% at light intensities of 27.5 and 68.8 mW/cm2. For reactions performed with the 365 nm LED source at light intensities of 5.12–512 mW/cm2, the peak yield of Iz was 34% at a light intensity of 51.2 mW/cm2. By varying the irradiation time, the maximum yield of Iz (34–35%) was obtained with irradiation times of 5–20 min using the 450 nm LED source at an intensity of 13.8 mW/cm2. Using the 365 nm LED source at an intensity of 25.6 mW/cm2, the maximum Iz yield obtained was 31% at irradiation times of 2–5 min. Thus, we obtained conditions that can provide an Iz yield of up to 35%.

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暴露于 LED 光后鸟嘌呤氧化损伤产物咪唑啉酮的产量增加

在 DNA 的碱基中，鸟嘌呤最容易被氧化。咪唑啉酮（Iz）是一种鸟嘌呤氧化损伤物，我们试图生成含 Iz 的低聚物。本文介绍了利用发射波长为 365 纳米和 450 纳米的发光二极管（LED）进行光氧化反应以提高 Iz 产率的方法和条件。在光照强度为 2.75-275 mW/cm2 时，使用 450 nm LED 光源进行光氧化反应，在光照强度为 27.5 和 68.8 mW/cm2 时，Iz 的峰值产率为 35%。使用 365 nm LED 光源在 5.12-512 mW/cm2 的光照强度下进行的反应，在光照强度为 51.2 mW/cm2 时，Iz 的峰值产量为 34%。通过改变辐照时间，使用 450 nm LED 光源，在光强为 13.8 mW/cm2 时，辐照时间为 5-20 分钟，Iz 的最大产量为 34-35%。使用强度为 25.6 mW/cm2 的 365 nm LED 光源，在照射时间为 2-5 分钟时，Iz 的最大产量为 31%。因此，我们获得的条件可以提供高达 35% 的 Iz 产率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Reactions

CiteScore

2.70

自引率

0.00%

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