Effect of 2,4-D and BAP on the Saurauia bracteosa In Vitro Culture

Rafaela Marlisa, R. Restiani, Aniek Prasetyaningsih, Cokorda Istri Meyga Semarayani
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Abstract

Saurauia bracteosa DC. (Pirdot) is one of the endemic woody plant species that has medicinal properties. The declining population in natural habitats caused this plant as a vulnerable species that need in vitro culture to prevent further extinction. The combination of 2.4-D and BAP as an exogenous PGR influenced the success of woody plant in vitro culture. Therefore, this research aimed to determine the effect of 2.4-D and BAP concentrations on the in vitro response of the S. bracteosa leaf explant. This research used a complete randomized design (CRD) with two factors: (1) the concentration of 2.4-D (0.50; 0.75; 1 mg/L), and (2) the concentration of BAP (B) (1; 2; 3 mg/L). This research consisted of 16 treatments (including a control); each treatment was repeated three times. All data were analyzed statistically using ANOVA, and the significant results will be followed by Duncan’s Multiple Range Test (DMRT). All PGR treatments could not produce a callus growth response, yet all explants showed various responses to browning intensity (0.08–1) and 100% browning. Further research still needs to be done to find the best type of explant and concentrations of PGR that can overcome the recalcitrant explant of Sauraia bracteosa. These results can be valuable information to promote the successful in vitro conservation of Sauraia bracteosa.
2,4-D 和 BAP 对苞片耧斗菜体外培养的影响
Saurauia bracteosa DC. (Pirdot) 是一种具有药用价值的特有木本植物。自然栖息地种群数量的减少导致该植物成为易危物种,需要进行离体培养以防止其进一步灭绝。2.4-D 和 BAP 作为外源 PGR 的组合影响了木本植物离体培养的成功率。因此,本研究旨在确定 2.4-D 和 BAP 浓度对苞叶榕叶片外植体离体反应的影响。本研究采用了完全随机设计(CRD),包含两个因素:(1) 2.4-D 的浓度(0.50;0.75;1 毫克/升),以及 (2) BAP (B) 的浓度(1;2;3 毫克/升)。这项研究包括 16 个处理(包括一个对照),每个处理重复三次。所有数据均采用方差分析进行统计分析,显著结果将采用邓肯多重范围检验(DMRT)。所有 PGR 处理都不能产生胼胝体生长反应,但所有外植体对褐变强度(0.08-1)和 100% 褐变都表现出不同的反应。要找到能克服苞叶石蒜顽固外植体的最佳外植体类型和 PGR 浓度,仍需进一步研究。这些结果对促进苞叶绍的成功离体保护具有重要价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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