{"title":"Methane fermentation to methanol (biological gas-to-liquid process) using Methylotuvimicrobium buryatense 5GB1C","authors":"Aradhana Priyadarsini, Kaustubh Chandrakant Khaire, Lepakshi Barbora, Subhrangsu Sundar Maitra, Vijayanand Suryakant Moholkar","doi":"10.1002/amp2.10172","DOIUrl":null,"url":null,"abstract":"<p>Methanol is a potential alternate liquid transportation fuel for blending with gasoline. Biochemical conversion of methane to methanol is a green process for methanol production. This paper reports biochemical methanol production using type I γ-proteobacteria <i>Methylotuvimicrobium buryatense</i>, which has particular importance from the viewpoint of scalable biological gas to liquid processes for industrial application. A statistical design of experiments (at the serum bottle level) was used to optimize fermentation parameters. Enhancement in methanol accumulation was attempted using methanol dehydrogenase inhibitors. This was followed by a validation experiment run in a bioreactor at optimum conditions. At optimum conditions (pH = 7, phosphate concentration = 140 mM, temperature = 25°C) and optical density (600 nm) of 0.3, a methanol titer of 8.54 mM was achieved in 24 h (methane conversion = 20.8%). The addition of a methanol dehydrogenase inhibitor (0.5 mM Ethylenediaminetetraacetic acid) enhanced the methanol concentration to 10.37 mM. Experiments in a 3.7 L bioreactor using 1.68 bar headspace pressure and optical density (600 nm) of 0.1 yielded 23.7 mM methanol in 24 h (methane conversion = 47.8%). The methanol titers obtained using <i>M. buryatense</i> 5GB1C in 24 h fermentation are significantly higher than several previously reported methanotrophs. These results demonstrate the potential of <i>M. buryatense</i> 5GB1C for the biochemical synthesis of methanol.</p>","PeriodicalId":87290,"journal":{"name":"Journal of advanced manufacturing and processing","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/amp2.10172","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of advanced manufacturing and processing","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/amp2.10172","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Methanol is a potential alternate liquid transportation fuel for blending with gasoline. Biochemical conversion of methane to methanol is a green process for methanol production. This paper reports biochemical methanol production using type I γ-proteobacteria Methylotuvimicrobium buryatense, which has particular importance from the viewpoint of scalable biological gas to liquid processes for industrial application. A statistical design of experiments (at the serum bottle level) was used to optimize fermentation parameters. Enhancement in methanol accumulation was attempted using methanol dehydrogenase inhibitors. This was followed by a validation experiment run in a bioreactor at optimum conditions. At optimum conditions (pH = 7, phosphate concentration = 140 mM, temperature = 25°C) and optical density (600 nm) of 0.3, a methanol titer of 8.54 mM was achieved in 24 h (methane conversion = 20.8%). The addition of a methanol dehydrogenase inhibitor (0.5 mM Ethylenediaminetetraacetic acid) enhanced the methanol concentration to 10.37 mM. Experiments in a 3.7 L bioreactor using 1.68 bar headspace pressure and optical density (600 nm) of 0.1 yielded 23.7 mM methanol in 24 h (methane conversion = 47.8%). The methanol titers obtained using M. buryatense 5GB1C in 24 h fermentation are significantly higher than several previously reported methanotrophs. These results demonstrate the potential of M. buryatense 5GB1C for the biochemical synthesis of methanol.