Optimization of Formulation for Development of Amaranth Incorporated Composite Fermented Milk Drink using Response Surface Methodology

Abstract

Background: Fermented milk products are highly nutritious food containing a significant amount of macro-nutrients, micro-nutrients as well as bioactive molecules which are required for the growth and overall development of all age’s people. However, they lack in fibre, vitamin C and essential mineral such as iron. Pseudo-cereal like amaranth is a principal source of energy consists of significant amount of carbohydrates, protein, fibre and some micronutrients viz., calcium, phosphorus, iron, zinc etc. Therefore, a study was undertaken to optimize the amaranth (Amaranthus cruentus) incorporated composite fermented milk drink (AICFMD). Methods: In present study, the central composite rotatory design (CCRD) with response surface methodology (RSM) was used to optimize the level of various ingredients (Independent variables) i.e. roasted amaranth flour, sugar and curd acidity to formulate the most acceptable AICFMD. Result: The results revealed that composite fermented milk drink prepared from toned milk 60.5%, amaranth 4%, sugar 10.5%, water 25.0% (w/w) with curd acidity 0.831% lactic acid and final total solid content maintained to 21.2%, resulted in good quality AICFMD.