MORPHOLOGICAL AND MOLECULAR STUDIES OF KAIS KINGFISH CYPRINION KAIS HECKEL, 1843 (PISCIES, CYPRINIFORMES, CYPRINIDAE) FROM THE MIDDLE OF IRAQ

Q4 Earth and Planetary Sciences

Bulletin of the Iraq Natural History Museum Pub Date : 2023-12-19 DOI:10.26842/binhm.7.2023.17.4.0611

Hind Dyia Hadi, Abed Hassan Baraaj, Atheer Hussain Ali

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Abstract

Cyprinidae species are the most abundant and widely distributed fish species in the inland waters of Iraq. Cyprinids are complex species, and it is difficult to identify them on the basis of morphology. Thus, the morphological characteristics must be achieved and confirmed by molecular analysis. Twenty specimens of Cyprinion kais Heckel, 1843 (Piscies, Cypriniformes, Cyprinidae) were collected from two localities at Tigris River in the middle of Iraq: five specimens from Al-Tharthar Lake, Saladin Province, and 15 specimens from Al- Zubaydiyah sub-district, Wasit Province. The DNA sequences of C. kais were done using the mitochondrial DNA cytochrome b (cytb) gene. After analysis, the sequences were compared with sequences of other fish genera and species available in GenBank. The barcoding result (DNA sequencing) showed that it fit with C. kais. We conclude the results of the current study confirm that the validity of the morphological diagnosis of C. kais, using DNA sequencing analysis.

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伊拉克中部 Kais kingfish cyprinion kais heckel, 1843 (piscies, cypriniformes, cyprinidae) 的形态和分子研究

鲤科鱼类是伊拉克内陆水域中数量最多、分布最广的鱼类物种。鲤科鱼类种类复杂，很难根据形态鉴定。因此，形态特征必须通过分子分析来实现和确认。本研究从伊拉克中部底格里斯河的两个地点采集了 20 个 Cyprinion kais Heckel, 1843（鱼纲，鲤形目，鲤科）标本：5 个标本来自萨拉丁省的 Al-Tharthar 湖，15 个标本来自瓦西特省的 Al- Zubaydiyah 分区。利用线粒体 DNA 细胞色素 b（cytb）基因对 C. kais 的 DNA 进行了测序。分析后，将序列与 GenBank 中其他鱼属和鱼种的序列进行了比较。条形码结果（DNA 测序）显示它与 C. kais 相符。我们总结说，本次研究的结果证实了利用 DNA 测序分析对 C. kais 进行形态学诊断的有效性。

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来源期刊

Bulletin of the Iraq Natural History Museum Environmental Science-Ecology

CiteScore

0.90

自引率

0.00%

发文量

审稿时长

10 weeks

期刊介绍： The Bulletin of the Iraq Natural History Museum, that''s affiliated with the Iraq Natural History Research Center and Museum / University of Baghdad, which founded in 1961 and is a peer reviewed, scientific open access journal, publishing original articles, article reviews, and case reports (short communication) in the natural history sciences. This journal is published twice times a year (Biannual). Bulletin of the Iraq Natural History Museum publishes 8-12 articles in each issue, according to the priority of manuscript acceptance. The variation in research areas for each issue is considered. The financial support of the Bulletin of the Iraq Natural History Museum comes from the publication fees paid by authors. No other financial supports are availablefor the Bulletin.