Nutrient Profiling, ICP-OES Mineral Analysis and UPLC Estimation of Amino Acids in Three Selected Ethnomedicinal Plants of North East India

Seema Khakhalary, Silistina Narzari
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Abstract

Background: Ethnomedicinal plants are rich traditional resources of Northeast India. Most of these plants are edible and are used as food by the tribal population of Northeast India. Quantitative nutritional analyses of three ethnomedicinal wild edible plant from Northeast India namely: Zanthoxylum oxyphyllum Edgew, Rotheca serrata (L.) Steane and Mabb. and Blumea lanceolaria (Roxb.) Druce were carried out to identify the occurrence and abundance of nutrients, minerals and amino acids. Methods: Proximate nutrients were estimated by standard AOAC methods. ICP-OES mineral analyzer was used to screen and quantify individual mineral elements. By performing UPLC analysis the presence and abundance of amino acids were investigated. Result: Proximate analysis revealed that the plant species contains adequate amounts of protein, fibre and ash. From nutritional perspectives, low fat and carbohydrate in these species specifies that they are desirable diet for human nutrition. In regard to mineral content, the plant species were rich in macro minerals K, Ca, Na and Mg. Heavy metals were found within the recommended dietary permissible limits for humans. All the essential and non essential amino acids excluding cysteine, methionine and histidine were present in reasonable quantities. The plant species may be exploited to provide quality diets to mitigate nutrient deficiency. Adding together its nutritional and medicinal properties it may potentially be utilized in therapeutics. The further scope of the research involves ascertaining their potentiality in food-based strategies to perk up nutritional health.
印度东北部三种精选民族药用植物的营养成分分析、ICP-OES 矿物分析和 UPLC 氨基酸估算
背景:民族药用植物是印度东北部丰富的传统资源。这些植物大多可食用,被印度东北部的部落居民用作食物。对印度东北部的三种药用野生食用植物(即 Zanthoxylum oxyphyllum Edgew、Zanthoxylum oxyphyllum Edgew 和 Zanthoxylum oxyphyllum Edgew)进行了定量营养分析:Zanthoxylum oxyphyllum Edgew、Rotheca serrata (L.) Steane and Mabb.和 Blumea lanceolaria (Roxb.) Druce)进行了定量营养分析,以确定营养成分、矿物质和氨基酸的含量和丰度。方法:采用 AOAC 标准方法估算近似养分。使用 ICP-OES 矿物分析仪筛选和量化单个矿物质元素。通过超高效液相色谱分析,研究了氨基酸的存在和丰度。结果近似分析表明,该植物品种含有充足的蛋白质、纤维和灰分。从营养学角度来看,这些物种的脂肪和碳水化合物含量较低,是人类理想的营养膳食。在矿物质含量方面,这些植物物种富含宏观矿物质 K、Ca、Na 和 Mg。重金属含量在建议的人类膳食允许范围内。除半胱氨酸、蛋氨酸和组氨酸外,所有必需和非必需氨基酸的含量都比较合理。该植物物种可用于提供优质膳食,以缓解营养缺乏症。再加上其营养和药用特性,它有可能被用于治疗。进一步的研究范围包括确定它们在基于食物的战略中的潜力,以改善营养健康。
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