Identification of cocoa (Theobroma cacao L.) genetic uniformity through RAPD molecular markers.

Pelita Perkebunan (a Coffee and Cocoa Research Journal) Pub Date : 2023-12-19 DOI:10.22302/iccri.jur.pelitaperkebunan.v39i3.566

Sulistyani Pancaningtyas, Nur Afni Helia Dewi, Mukhamad Su’udi

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Abstract

Theobroma cacao L. has been propagated through either generative or vegetative techniques. One of the vegetative methods of propagating cocoa is somatic embryogenesis. Somatic embryogenesis has been employed by the Indonesian Coffee and Cocoa Research Institute for producing cocoa seedlings. Plant breeding activities are hampered by the significant level of plant heterogeneity among their progeny. Mislabelling of genetic impurities can be an issue. Molecular markers can be used to detect genetic variation at an early stage. The most common marker is the RAPD molecular marker. The study aims to determine the polymorphic RAPD primers in the analysis of genetic uniformity between mother plants and the seedlings derived from somatic embryogenesis (SE). The analyzed samples consisted of twelve individuals: six mother plants and six seedlings derived from SE. The results revealed that the percentage of polymorphic bands was 100% with band sizes ranging from 295-2785 bp for primer GY169 while for primer GY107 percentage of polymorphic bands was 80% with band sizes ranging from 345-1678 bp. Primer GY169 and primer GY107 can be amplified and used for cocoa similarity and heterogeneity.

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通过 RAPD 分子标记鉴定可可（Theobroma cacao L. ）的遗传一致性。

可可豆可通过产生或无性繁殖技术进行繁殖。体细胞胚胎发生是无性繁殖可可的方法之一。印度尼西亚咖啡和可可研究所采用体细胞胚胎发生法培育可可幼苗。植物育种活动因其后代之间的植物异质性程度很高而受到阻碍。基因杂质的错误标记可能是一个问题。分子标记可用于早期检测遗传变异。最常见的标记是 RAPD 分子标记。本研究旨在确定多态 RAPD 引物，用于分析母株与体细胞胚胎发生（SE）产生的幼苗之间的遗传一致性。分析的样本包括 12 个个体：6 个母株和 6 个由体细胞胚胎发生（SE）产生的幼苗。结果显示，引物 GY169 的多态性条带比例为 100%，条带大小为 295-2785 bp；引物 GY107 的多态性条带比例为 80%，条带大小为 345-1678 bp。引物 GY169 和引物 GY107 可以扩增并用于检测可可的相似性和异质性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Pelita Perkebunan (a Coffee and Cocoa Research Journal)

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