ANTIMYCOTIC ACTIVITY OF THE ISOLATES OF LACTOBACTERIA FROM WA-TER AND MUSSELS OF BLACK SEA

Microbiology&Biotechnology Pub Date : 2023-12-20 DOI:10.18524/2307-4663.2023.3(59).287048

A. Merlich, I. I. Kimurzhyi, R. R. Kovalchuk, M. V. Shutylo, V. Ivanytsia

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Abstract

Aim. Determination of the presence of antimycotic activity and its level in thirteen lactobacteria isolates from Black Sea water and mussels. Methods. Antimycotic activity of lactic acid bacteria (LAB) isolates was tested by agar diffusion method in 24-well plates. To determine character of action the plates were incubated for 7 days at 25 °C. Results. For the first time antimycotic activity of marine LAB from Odesa coast was estimated. The majority of the tested isolates of lactobateria exhibited antimycotic activity of high level completely inhibiting (in 100%) as mycelium growth as well as spore formation of Penicillium expansum UKM F-575 and Aspergillus niger UKM F-16706 on second day of the study. Four isolates (M.5.1, M.5.2, M.7.1, M.7.2) showed lower antimycotic activity (from 0 to 75%). Eight LAB isolates from seawater (W.1.1, W.1.2, W.1.3, W.1.4, W.1.5, W.1.дк, W.2.3, W.2.4) and one isolate from mussel liquor (M4.1) completely inhibited mycelial growth and sporulation of P. expansum UКМ F-575 and A. niger UКМ F-16706 even within seven days indicating fungicidal character of action. Conclusions. The most of tested LAB isolates from Black Sea exhibited high antimycotic activity against P. expansum UКМ F-575 and A. niger UКМ F-16706 indicator strains. The most active lactobacteria isolates were W.1.1, W.1.2, W.1.3, W.1.4, W.1.5, W.1.дк, W.2.3, W.2.4 isolated from Black Sea water and M4.1 – from mussel liquor. The character of the revealed antimycotic activity was determined as fungicidal.

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从黑海腰带和贻贝中分离出的乳酸菌的抗霉菌活性

目的测定从黑海海水和贻贝中分离出的 13 种乳酸菌的抗霉菌活性及其水平。方法。在 24 孔板中采用琼脂扩散法检测乳酸菌（LAB）分离物的抗霉菌活性。为确定作用特征，将平板在 25 °C 下培养 7 天。结果。首次评估了敖德萨海岸海洋 LAB 的抗霉菌活性。大多数受测乳杆菌分离物在研究第二天表现出高水平的抗真菌活性，完全抑制（100%）扩张青霉 UKM F-575 和黑曲霉 UKM F-16706 的菌丝生长和孢子形成。四个分离株（M.5.1、M.5.2、M.7.1、M.7.2）的抗霉菌活性较低（从 0% 到 75%）。海水中的八个 LAB 分离物（W.1.1、W.1.2、W.1.3、W.1.4、W.1.5、W.1.дк、W.2.3、W.2.4）和贻贝液中的一种分离物（M4.1）在七天内完全抑制了扩张菌 UКМ F-575 和黑曲霉 UКМ F-16706 的菌丝生长和孢子产生，这表明它们具有杀菌作用。结论来自黑海的大多数经测试的乳杆菌分离物对扩张菌 UКМ F-575 和黑曲霉 UКМ F-16706 指示菌株具有很高的抗真菌活性。最活跃的乳酸菌分离菌株是分离自黑海海水的 W.1.1、W.1.2、W.1.3、W.1.4、W.1.5、W.1.дк、W.2.3、W.2.4 和分离自贻贝液的 M4.1。所发现的抗真菌活性被确定为杀真菌性。

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Microbiology&Biotechnology

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