Physiological and Transcriptional Analysis Provides Insights into Tea Saponin Biosynthesis and Regulation in Response to SA in Camellia vietnamensis Huang

IF 3.1 3区农林科学 Q1 HORTICULTURE

Horticulturae Pub Date : 2023-12-20 DOI:10.3390/horticulturae10010008

Yang Li, Heqin Yan, Muhammad Zeeshan Ul Haq, Ya Liu, You-gen Wu, Jing Yu, Pengguo Xia

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引用次数: 0

Abstract

Camellia vietnamensis Huang is an important and famous woody oil crop with high economic value in China because of its high-quality, edible, and medicinal oil. As one of its major active components, tea saponin (triterpenoid saponin) has shown anticancer, antioxidant, bacteriostatic, and other pharmacological activities. In this study, C. vietnamensis was used as an experimental material to determine the tea saponin content and physiological activity indicators after salicylic acid (SA) treatment and to analyze the differential expression genes of key metabolic pathways in response to SA by combining transcriptome data. The results showed that SA treatment increased the content of tea saponin and total phenols in leaves; effectively promoted the activity of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX); and decreased the content of malondialdehyde (MDA). A total of 60,038 genes, including 5871 new genes, were obtained by the RNA-seq. There were 6609 significantly differential expression genes mainly enriched in pathways such as sesquiterpenoid and triterpenoid biosynthesis, terpenoid backbone biosynthesis, diterpenoid biosynthesis, and flavonoid biosynthesis. The SA-induced key structural genes (SQS, SQE, bAS, CYP450, and UGT) and transcription factors related to the tea saponin biosynthetic pathway were screened by weighted gene co-expression network analysis (WGCNA). The results of this study could provide a theoretical basis and a new technical method to improve the content of tea saponin, with its excellent anticancer activity, in C. vietnamensis.

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生理和转录分析为了解黄山茶皂苷的生物合成和对 SA 的调控提供了见解

黄山茶是中国重要的著名木本油料作物，因其油质优良、可食用、可药用而具有很高的经济价值。作为其主要活性成分之一的茶皂素（三萜类皂素）具有抗癌、抗氧化、抑菌等药理活性。本研究以越南越橘为实验材料，测定水杨酸（SA）处理后茶皂素含量和生理活性指标，并结合转录组数据分析关键代谢途径基因对SA的差异表达。结果表明，SA处理提高了叶片中茶皂素和总酚的含量；有效促进了超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX）的活性；降低了丙二醛（MDA）的含量。通过 RNA-seq 共获得了 60,038 个基因，其中包括 5871 个新基因。其中有 6609 个基因有明显的表达差异，主要富集在倍半萜和三萜类生物合成、萜类骨架生物合成、二萜类生物合成和黄酮类生物合成等途径中。通过加权基因共表达网络分析（WGCNA）筛选了SA诱导的与茶皂素生物合成途径相关的关键结构基因（SQS、SQE、bAS、CYP450和UGT）和转录因子。研究结果为提高越南越橘茶皂素含量提供了理论依据和新的技术方法，越南越橘茶皂素具有良好的抗癌活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Horticulturae HORTICULTURE-

CiteScore

3.50

自引率

19.40%

发文量

998

期刊介绍： Horticulturae (ISSN 2311-7524) is an international, multidisciplinary, peer-reviewed, open access journal focusing on all areas and aspects of temperate to tropical horticulture. It publishes original empirical and theoretical research articles, short communications, reviews, and opinion articles. We intend to encourage scientists to publish and communicate their results concerning all branches of horticulture in a timely manner and in an open venue, after being evaluated by the journal editors and randomly selected independent expert reviewers, so that all articles will never be judged in relation to how much they confirm or criticize the opinions of other researchers.