THE COMPARISON OF SIDEROPHORES CONTENT IN BACTERIA ISOLATED FROM BLACK SEA MUSSELS

Microbiology&Biotechnology Pub Date : 2023-12-20 DOI:10.18524/2307-4663.2023.3(59).287367

S. A. Martynenko, M. O. Finogenova, A. Semenets, M. Galkin

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Abstract

Aim. This research was carried out for determination of ability to produce siderophores by strains of Pseudomonas aeruginosa and Bacillus sp. isolated from Black Sea mussels and for studying characteristics of their synthesis at mono- and co-cultivation of researched strains. Materials and methods. In the study we used four strains of Pseudomonas aeruginosa and two strains of Bacillus sp. Monocultivation and co-cultivation were carried out with these strains on a LB medium. CAS (chrome azurol S) analysis was used to determine the content of synthesized siderophores, the measurement was carried out in spectrophotometer SmartSpec Plus at 630 nm. Conclusions. This study showed that marine strains of P. aeruginosa can produce more siderophores than marine strains of Bacillus sp. At monocultivation, strain P. aeruginosa М1 was able to produce the largest amount of siderophores with value of SU (siderophores units) 65 ± 4% and the smallest one strain B. atrophaeus МН4 with value of SU 21 ± 1%. Co-cultivation provides an increase in production of siderophores in each strain, that is the result of special interactions between different microorganisms. And through it, the combination B. subtilis MC3+P. aeruginosa M1 demonstrated the highest content of siderophores with value of SU 81 ± 6%, the lowest content was shown by combination B. atrophaeus МН4+P. aeruginosa M3 with value of SU 41 ± 4%. And such results showed that co-cultivation is the useful method for obtaining more content of siderophores from already famous strains.

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从黑海贻贝中分离出的细菌中嗜苷酸含量的比较

研究目的本研究旨在确定从黑海贻贝中分离出来的铜绿假单胞菌和芽孢杆菌菌株产生嗜苷酸盐的能力，并研究这些菌株在单一培养和联合培养时合成嗜苷酸盐的特点。材料和方法。在研究中，我们使用了四株铜绿假单胞菌和两株芽孢杆菌。CAS（铬唑啉 S）分析用于确定合成嗜苷酸盐的含量，测量在分光光度计 SmartSpec Plus 上进行，波长为 630 nm。结论该研究表明，海洋铜绿微囊藻菌株比海洋芽孢杆菌菌株能产生更多的嗜苷酸盐。在单一培养条件下，菌株铜绿微囊藻М1能产生最多的嗜苷酸盐，SU（嗜苷酸盐单位）值为 65 ± 4%，而菌株芽孢杆菌МН4产生的嗜苷酸盐最少，SU 值为 21 ± 1%。联合培养能提高每个菌株嗜苷酸盐的产量，这是不同微生物之间特殊相互作用的结果。通过联合培养，枯草芽孢杆菌 MC3+ 铜绿微囊藻 M1 的嗜苷酸含量最高，为 SU 81 ± 6%，而阿特罗非斯芽孢杆菌 МН4+ 铜绿微囊藻 M3 的嗜苷酸含量最低，为 SU 41 ± 4%。这些结果表明，联合培养是从著名菌株中获得更多嗜苷酸含量的有效方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Microbiology&Biotechnology

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