Advances in microfluidic-based DNA methylation analysis

IF 2.7

Nanotechnology and Precision Engineering Pub Date : 2023-12-21 DOI:10.1063/10.0023845

Jiwen Li, Tiechuan Li, Xuexin Duan

{"title":"Advances in microfluidic-based DNA methylation analysis","authors":"Jiwen Li, Tiechuan Li, Xuexin Duan","doi":"10.1063/10.0023845","DOIUrl":null,"url":null,"abstract":"DNA methylation has been extensively investigated in recent years, not least because of its known relationship with various diseases. Progress in analytical methods can greatly increase the relevance of DNA methylation studies to both clinical medicine and scientific research. Microfluidic chips are excellent carriers for molecular analysis, and their use can provide improvements from multiple aspects. On-chip molecular analysis has received extensive attention owing to its advantages of portability, high throughput, low cost, and high efficiency. In recent years, the use of novel microfluidic chips for DNA methylation analysis has been widely reported and has shown obvious superiority to conventional methods. In this review, we first focus on DNA methylation and its applications. Then, we discuss advanced microfluidic-based methods for DNA methylation analysis and describe the great progress that has been made in recent years. Finally, we summarize the advantages that microfluidic technology brings to DNA methylation analysis and describe several challenges and perspectives for on-chip DNA methylation analysis. This review should help researchers improve their understanding and make progress in developing microfluidic-based methods for DNA methylation analysis.","PeriodicalId":87330,"journal":{"name":"Nanotechnology and Precision Engineering","volume":"29 5","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nanotechnology and Precision Engineering","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1063/10.0023845","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

DNA methylation has been extensively investigated in recent years, not least because of its known relationship with various diseases. Progress in analytical methods can greatly increase the relevance of DNA methylation studies to both clinical medicine and scientific research. Microfluidic chips are excellent carriers for molecular analysis, and their use can provide improvements from multiple aspects. On-chip molecular analysis has received extensive attention owing to its advantages of portability, high throughput, low cost, and high efficiency. In recent years, the use of novel microfluidic chips for DNA methylation analysis has been widely reported and has shown obvious superiority to conventional methods. In this review, we first focus on DNA methylation and its applications. Then, we discuss advanced microfluidic-based methods for DNA methylation analysis and describe the great progress that has been made in recent years. Finally, we summarize the advantages that microfluidic technology brings to DNA methylation analysis and describe several challenges and perspectives for on-chip DNA methylation analysis. This review should help researchers improve their understanding and make progress in developing microfluidic-based methods for DNA methylation analysis.

查看原文本刊更多论文

基于微流控 DNA 甲基化分析的进展

近年来，DNA 甲基化已被广泛研究，这主要是因为它与各种疾病的关系众所周知。分析方法的进步可以大大提高 DNA 甲基化研究与临床医学和科学研究的相关性。微流控芯片是分子分析的绝佳载体，使用它可以从多个方面进行改进。芯片分子分析因其便携、高通量、低成本和高效率等优势而受到广泛关注。近年来，利用新型微流控芯片进行 DNA 甲基化分析的研究已被广泛报道，并显示出明显优于传统方法的优势。在这篇综述中，我们首先关注 DNA 甲基化及其应用。然后，我们讨论了基于微流控芯片的先进 DNA 甲基化分析方法，并介绍了近年来取得的巨大进步。最后，我们总结了微流控技术为 DNA 甲基化分析带来的优势，并描述了片上 DNA 甲基化分析面临的几个挑战和前景。这篇综述应能帮助研究人员加深理解并在开发基于微流控技术的 DNA 甲基化分析方法方面取得进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Nanotechnology and Precision Engineering

自引率

0.00%

发文量