Perfluorooctanoic Acid (PFOA) Exposure Compromises Fertility by Affecting Ovarian and Oocyte Development

IF 4.9 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Molecular Sciences Pub Date : 2023-12-21 DOI:10.3390/ijms25010136

Han Zhang, Lulu Han, Lijun Qiu, Bo Zhao, Yang Gao, Z. Chu, Xiaoxin Dai

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引用次数: 0

Abstract

PFOA, a newly emerging persistent organic pollutant, is widely present in various environmental media. Previous reports have proved that PFOA exposure can accumulate in the ovary and lead to reproductive toxicity in pregnant mice. However, the potential mechanism of PFOA exposure on fertility remains unclear. In this study, we explore how PFOA compromises fertility in the zebrafish. The data show that PFOA (100 mg/L for 15 days) exposure significantly impaired fertilization and hatching capability. Based on tissue sections, we found that PFOA exposure led to ovarian damage and a decrease in the percentage of mature oocytes. Moreover, through in vitro incubation, we determined that PFOA inhibits oocyte development. We also sequenced the transcriptome of the ovary of female zebrafish and a total of 284 overlapping DEGs were obtained. Functional enrichment analysis showed that 284 overlapping DEGs function mainly in complement and coagulation cascades signaling pathways. In addition, we identified genes that may be associated with immunity, such as LOC108191474 and ZGC:173837. We found that exposure to PFOA can cause an inflammatory response that can lead to ovarian damage and delayed oocyte development.

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全氟辛酸（PFOA）暴露会影响卵巢和卵母细胞的发育，从而损害生育能力

PFOA 是一种新出现的持久性有机污染物，广泛存在于各种环境介质中。以往的报告证明，接触全氟辛酸会在妊娠小鼠的卵巢中蓄积，并导致生殖毒性。然而，PFOA 暴露对生育能力的潜在机制仍不清楚。在本研究中，我们探讨了 PFOA 如何损害斑马鱼的生育能力。数据显示，暴露于 PFOA（100 毫克/升，15 天）会显著损害受精和孵化能力。根据组织切片，我们发现暴露于 PFOA 会导致卵巢损伤和成熟卵母细胞比例下降。此外，通过体外培养，我们确定 PFOA 会抑制卵母细胞的发育。我们还对雌性斑马鱼卵巢的转录组进行了测序，共获得了284个重叠的DEGs。功能富集分析表明，284 个重叠 DEGs 主要在补体和凝血级联信号通路中发挥作用。此外，我们还发现了可能与免疫相关的基因，如 LOC108191474 和 ZGC:173837。我们发现，暴露于 PFOA 可引起炎症反应，导致卵巢损伤和卵母细胞发育延迟。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Molecular Sciences Chemistry-Organic Chemistry

CiteScore

8.10

自引率

10.70%

发文量

13472

审稿时长

17.49 days

期刊介绍： The International Journal of Molecular Sciences (ISSN 1422-0067) provides an advanced forum for chemistry, molecular physics (chemical physics and physical chemistry) and molecular biology. It publishes research articles, reviews, communications and short notes. Our aim is to encourage scientists to publish their theoretical and experimental results in as much detail as possible. Therefore, there is no restriction on the length of the papers or the number of electronics supplementary files. For articles with computational results, the full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material (including animated pictures, videos, interactive Excel sheets, software executables and others).