Tracking reproductive events: Hoof growth and steroid hormone concentrations in hair and hoof tissues in moose (Alces alces).

IF 2.6 3区 环境科学与生态学 Q2 BIODIVERSITY CONSERVATION
Conservation Physiology Pub Date : 2023-12-13 eCollection Date: 2023-01-01 DOI:10.1093/conphys/coad097
Mandy J Keogh, Daniel P Thompson, John A Crouse
{"title":"Tracking reproductive events: Hoof growth and steroid hormone concentrations in hair and hoof tissues in moose (<i>Alces alces</i>).","authors":"Mandy J Keogh, Daniel P Thompson, John A Crouse","doi":"10.1093/conphys/coad097","DOIUrl":null,"url":null,"abstract":"<p><p>Measurements of reproductive and stress-related hormones in keratinous tissues (e.g. hair, claws, hooves, baleen) can provide a record of stress and reproductive response in wildlife. We evaluated a method to collect keratin tissue from hooves of immobilized moose (<i>Alces alces</i>) and validated enzyme immunoassays for measuring cortisol and progesterone in hooves and hair. We also measured the annual growth and wear rates of moose hooves. Progesterone (range: 1.0-43.7 pg/mg) and cortisol (range: 0.05-2.9 pg/mg) were measurable and showed variation among hoof samples and moose. Pregnant females had twice as high progesterone concentrations (18.00 ± 3.73 pg/mg) from hoof sample locations post breeding compared to non-pregnant moose (9.40 ± 0.25 pg/mg). Annual hoof growth differed between the front (5.58 ± 0.12 cm) and rear (4.73 ± 0.13 cm) hooves and varied by season with higher growth rates during summer which decreased into autumn and winter. Adult female hooves represented between 1.6 and 2.1 years of growth and included up to two reproductive cycles. We established a method to estimate hoof growth rate and applied this to postmortem samples and were able to detect previous pregnancies. Shoulder guard hairs grew between August and March including during late gestation; however, hair progesterone concentrations (range: 2-107.1 pg/mg) were not related to reproductive state. Hair cortisol concentrations in our study (range: 0.2-15.9 pg/mg) were within the range of values previously reported for cervids. Our study supports the use of hooves for longitudinal sampling and measuring reproductive and stress-related hormones, providing a new tool for tracking reproductive events and understanding what variables may contribute to population level changes in reproduction.</p>","PeriodicalId":54331,"journal":{"name":"Conservation Physiology","volume":"11 1","pages":"coad097"},"PeriodicalIF":2.6000,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10722880/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Conservation Physiology","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1093/conphys/coad097","RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"BIODIVERSITY CONSERVATION","Score":null,"Total":0}
引用次数: 0

Abstract

Measurements of reproductive and stress-related hormones in keratinous tissues (e.g. hair, claws, hooves, baleen) can provide a record of stress and reproductive response in wildlife. We evaluated a method to collect keratin tissue from hooves of immobilized moose (Alces alces) and validated enzyme immunoassays for measuring cortisol and progesterone in hooves and hair. We also measured the annual growth and wear rates of moose hooves. Progesterone (range: 1.0-43.7 pg/mg) and cortisol (range: 0.05-2.9 pg/mg) were measurable and showed variation among hoof samples and moose. Pregnant females had twice as high progesterone concentrations (18.00 ± 3.73 pg/mg) from hoof sample locations post breeding compared to non-pregnant moose (9.40 ± 0.25 pg/mg). Annual hoof growth differed between the front (5.58 ± 0.12 cm) and rear (4.73 ± 0.13 cm) hooves and varied by season with higher growth rates during summer which decreased into autumn and winter. Adult female hooves represented between 1.6 and 2.1 years of growth and included up to two reproductive cycles. We established a method to estimate hoof growth rate and applied this to postmortem samples and were able to detect previous pregnancies. Shoulder guard hairs grew between August and March including during late gestation; however, hair progesterone concentrations (range: 2-107.1 pg/mg) were not related to reproductive state. Hair cortisol concentrations in our study (range: 0.2-15.9 pg/mg) were within the range of values previously reported for cervids. Our study supports the use of hooves for longitudinal sampling and measuring reproductive and stress-related hormones, providing a new tool for tracking reproductive events and understanding what variables may contribute to population level changes in reproduction.

跟踪繁殖事件:驼鹿(Alces alces)毛发和蹄组织中的蹄生长和类固醇激素浓度。
测量角质组织(如毛发、爪、蹄、须)中与生殖和压力相关的激素可以记录野生动物的压力和生殖反应。我们评估了一种从固定的驼鹿(Alces alces)蹄中收集角蛋白组织的方法,并验证了测量蹄和毛发中皮质醇和孕酮的酶免疫测定法。我们还测量了驼鹿蹄的年生长率和磨损率。孕酮(范围:1.0-43.7 pg/mg)和皮质醇(范围:0.05-2.9 pg/mg)均可测量,并且在蹄样本和驼鹿之间存在差异。与未怀孕的驼鹿(9.40 ± 0.25 pg/mg)相比,怀孕的雌性驼鹿在繁殖后的蹄部样本中的孕酮浓度(18.00 ± 3.73 pg/mg)是未怀孕的驼鹿的两倍。前蹄(5.58 ± 0.12 厘米)和后蹄(4.73 ± 0.13 厘米)的年蹄生长率不同,季节也不同,夏季蹄生长率较高,秋冬季节蹄生长率下降。成年雌性蹄的生长期为 1.6 至 2.1 年,最多包括两个繁殖周期。我们建立了一种估算蹄生长率的方法,并将其应用到死后样本中,从而能够检测出以前的怀孕情况。护肩毛在八月到三月之间生长,包括在妊娠晚期;但是,毛发孕酮浓度(范围:2-107.1 pg/mg)与繁殖状态无关。在我们的研究中,毛发的皮质醇浓度(范围:0.2-15.9 pg/mg)与之前报告的颈鹿皮质醇浓度范围一致。我们的研究支持使用蹄进行纵向采样,并测量与生殖和压力相关的激素,为跟踪生殖事件和了解哪些变量可能会导致种群水平的生殖变化提供了一种新工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Conservation Physiology
Conservation Physiology Environmental Science-Management, Monitoring, Policy and Law
CiteScore
5.10
自引率
3.70%
发文量
71
审稿时长
11 weeks
期刊介绍: Conservation Physiology is an online only, fully open access journal published on behalf of the Society for Experimental Biology. Biodiversity across the globe faces a growing number of threats associated with human activities. Conservation Physiology will publish research on all taxa (microbes, plants and animals) focused on understanding and predicting how organisms, populations, ecosystems and natural resources respond to environmental change and stressors. Physiology is considered in the broadest possible terms to include functional and mechanistic responses at all scales. We also welcome research towards developing and refining strategies to rebuild populations, restore ecosystems, inform conservation policy, and manage living resources. We define conservation physiology broadly and encourage potential authors to contact the editorial team if they have any questions regarding the remit of the journal.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信