[Evaluation of Sysmex UF-5000 flow cytometer flag BACT-info for Gram discrimination in urinary tract infection].

IF 1.9 4区 医学 Q4 MICROBIOLOGY
Revista Espanola De Quimioterapia Pub Date : 2024-02-01 Epub Date: 2023-12-11 DOI:10.37201/req/063.2023
I Angulo-López, D Martín-Corujo, J L Díaz-de-Tuesta Del Arco, L García de Guadiana-Romualdo, M Basaras-Ibarzabal
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引用次数: 0

Abstract

Objective: Urine culture as a gold standard for the diagnosis of urinary tract infection (UTI) involves a considerable workload in Clinical Microbiology Departments, due to the high number of samples received that will ultimately be negative. Therefore, it is necessary to use screening systems that also reduce the turnaround time for UTI diagnosis. The new flow cytometer UF-5000 (Sysmex Corporation) is able to differentiate between Gram-negative and Gram-positive bacteria using the BACT-info parameter according to manufacturer. The aim of our study was to evaluate the gram discrimination ability of the UF-5000 cytometer.

Methods: A prospective study with 449 urine samples collected consecutively was conducted, in the period 7/3/2022-27/5/2022, in which the BACT-info flag was compared with urine culture as the reference method.

Results: The sensitivity obtained for both Gram-negative and Gram-positive bacteria was above 95%. However, for Gram-positive bacteria, the moderate Kappa index (0.49) and the low positive predictive value (37.1%) indicated that the correlation between BACT-info flag and urine culture was not acceptable and should not be reported to the requesting clinician.

Conclusions: Implementation of the third generation UF-5000 cytometer represents a significant advance in the aetiological orientation of UTIs caused by Gram-negative bacteria. Reporting the Gram morphology in the urine samples reduces the response time in the microbiological diagnosis of UTI, which would have an impact on the reduction and optimisation of empirical treatment, and thus on the generation of antimicrobial resistance.

[评估 Sysmex UF-5000 流式细胞仪旗标 BACT-info 在尿路感染中的革兰氏分辨能力]。
目的:尿培养是诊断尿路感染(UTI)的金标准,由于收到的大量样本最终会呈阴性,临床微生物学部门的工作量相当大。因此,有必要使用能缩短UTI 诊断周转时间的筛查系统。新型流式细胞仪 UF-5000(Sysmex 公司)能根据制造商提供的 BACT-info 参数区分革兰氏阴性菌和革兰氏阳性菌。我们的研究旨在评估 UF-5000 流式细胞仪的革兰氏鉴别能力:方法:对 2022 年 3 月 7 日至 5 月 27 日期间连续采集的 449 份尿液样本进行了前瞻性研究,将 BACT-info 标志与作为参考方法的尿液培养进行了比较:结果:对革兰氏阴性菌和革兰氏阳性菌的灵敏度均高于 95%。然而,对于革兰氏阳性菌,中等程度的卡帕指数(0.49)和较低的阳性预测值(37.1%)表明,BACT-info 标志与尿培养之间的相关性不可接受,不应向提出要求的临床医生报告:结论:第三代 UF-5000 细胞计数器的使用标志着在革兰氏阴性菌引起的尿路感染病因定位方面取得了重大进展。报告尿液样本中的革兰氏形态缩短了UTI微生物诊断的响应时间,这将有助于减少和优化经验性治疗,从而减少抗菌药耐药性的产生。
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来源期刊
CiteScore
2.90
自引率
10.50%
发文量
146
审稿时长
>12 weeks
期刊介绍: The official journal of the Sociedad Española de Quimioterapia (Spanish Society of Chemotherapy), publishes articles that further knowledge and advance the science and application of antimicrobial chemotherapy with antibiotics and antifungal, antiviral and antiprotozoal agents primarily in human medicine. Authors sign an exclusive license agreement, where authors have copyright but license exclusive rights in their article to the Publisher. All manuscripts are free open access. Revista Española de Quimioterapia includes the following sections: reviews, original articles, brierf reports, letters, and consensus documents.
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