Rapid Immunohistochemistry Based on Ultrasonic Thermal Steam Heating for Improvement of Intraoperative Diagnosis.

IF 1.3 4区医学 Q3 ANATOMY & MORPHOLOGY

Applied Immunohistochemistry & Molecular Morphology Pub Date : 2024-03-01 Epub Date: 2023-12-11 DOI:10.1097/PAI.0000000000001178

Guangyin Yu, Lili Tao, Mengying Liao, ChuQiang Huang, Cuimin Chen, Xiaomin Yin

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引用次数: 0

Abstract

Objectives: To evaluate the role of rapid immunohistochemistry (RIHC) based on ultrasonic thermal steam heating in improving diagnostic accuracy of intraoperative frozen section diagnosis and to recommend RIHC antibody panels for pathologic differential diagnosis.

Materials and methods: RIHC based on ultrasonic thermal steam heating was tested for intraoperative frozen diagnosis with difficulty in diagnosis, and all slides were reviewed and compared with the final diagnosis. Ninety-three cases of surgical specimens involving RIHC examination were studied. Discordance rates with paraffin immunohistochemistry were calculated.

Results: In 93 cases where RIHC was performed, 85 cases (91%) were proven to be helpful for the diagnosis. A total of 58 antibodies were used for RIHC 276 times, of which 19 antibodies were not effective 25 times. Fifteen RIHC antibody panels are recommended based on staining stability and utilization frequency.

Conclusion: After improving the staining method, ultrasonic thermal steam heating RIHC is practical, convenient, and cost-effective, making it suitable for use in any pathology department with routine immunohistochemistry reagents. It plays an important auxiliary role in improving the accuracy of intraoperative rapid pathologic diagnosis.

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基于超声热蒸汽加热的快速免疫组化技术用于改进术中诊断

目的评估基于超声热蒸汽加热的快速免疫组化（RIHC）在提高术中冰冻切片诊断准确性方面的作用，并推荐用于病理鉴别诊断的RIHC抗体库：对诊断困难的术中冰冻切片诊断进行基于超声热蒸汽加热的 RIHC 试验，对所有切片进行复查并与最终诊断进行比较。研究了 93 例涉及 RIHC 检查的手术标本。结果：结果：在93例进行了RIHC检查的病例中，有85例（91%）被证明有助于诊断。RIHC 共使用了 58 种抗体 276 次，其中 19 种抗体无效 25 次。根据染色稳定性和使用频率，推荐使用 15 种 RIHC 抗体：结论：改进染色方法后的超声热蒸汽加热 RIHC 实用、方便、经济，适合任何病理科使用常规免疫组化试剂。它在提高术中快速病理诊断的准确性方面发挥了重要的辅助作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Applied Immunohistochemistry & Molecular Morphology ANATOMY & MORPHOLOGY-MEDICAL LABORATORY TECHNOLOGY

CiteScore

3.20

自引率

0.00%

发文量

153

期刊介绍： Applied Immunohistochemistry & Molecular Morphology covers newly developed identification and detection technologies, and their applications in research and diagnosis for the applied immunohistochemist & molecular Morphologist. Official Journal of the International Society for Immunohistochemisty and Molecular Morphology.