Absolute retinal blood flow in healthy eyes and in eyes with retinal vein occlusion

IF 2.9 4区医学 Q2 PERIPHERAL VASCULAR DISEASE

Microvascular research Pub Date : 2023-12-18 DOI:10.1016/j.mvr.2023.104648

Thibaud Mautuit , Pierre Cunnac , Frédéric Truffer , André Anjos , Rebecca Dufrane , Gilbert Maître , Martial Geiser , Christophe Chiquet

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引用次数: 0

Abstract

Purpose

To measure non-invasively retinal venous blood flow (RBF) in healthy subjects and patients with retinal venous occlusion (RVO).

Methods

The prototype named AO-LDV (Adaptive Optics Laser Doppler Velocimeter), which combines a new absolute laser Doppler velocimeter with an adaptive optics fundus camera (rtx1, Imagine Eyes®, Orsay, France), was studied for the measurement of absolute RBF as a function of retinal vessel diameters and simultaneous measurement of red blood cell velocity. RBF was measured in healthy subjects (n = 15) and patients with retinal venous occlusion (RVO, n = 6). We also evaluated two softwares for the measurement of retinal vessel diameters: software 1 (automatic vessel detection, profile analysis) and software 2 (based on the use of deep neural networks for semantic segmentation of vessels, using a M2u-Net architecture).

Results

Software 2 provided a higher rate of automatic retinal vessel measurement (99.5 % of 12,320 AO images) than software 1 (64.9 %) and wider measurements (75.5 ± 15.7 μm vs 70.9 ± 19.8 μm, p < 0.001). For healthy subjects (n = 15), all the retinal veins in one eye were measured to obtain the total RBF. In healthy subjects, the total RBF was 37.8 ± 6.8 μl/min. There was a significant linear correlation between retinal vessel diameter and maximal velocity (slope = 0.1016; p < 0.001; r² = 0.8597) and a significant power curve correlation between retinal vessel diameter and blood flow (3.63 × 10⁻⁵ × D^2.54; p < 0.001; r² = 0.7287). No significant relationship was found between total RBF and systolic and diastolic blood pressure, ocular perfusion pressure, heart rate, or hematocrit. For RVO patients (n = 6), a significant decrease in RBF was noted in occluded veins (3.51 ± 2.25 μl/min) compared with the contralateral healthy eye (11.07 ± 4.53 μl/min). For occluded vessels, the slope between diameter and velocity was 0.0195 (p < 0.001; r² = 0.6068) and the relation between diameter and flow was Q = 9.91 × 10⁻⁶ × D^2.41 (p < 0.01; r² = 0.2526).

Conclusion

This AO-LDV prototype offers new opportunity to study RBF in humans and to evaluate treatment in retinal vein diseases.

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健康眼睛和视网膜静脉闭塞眼睛的绝对视网膜血流量

目的对健康受试者和视网膜静脉闭塞（RVO）患者的视网膜静脉血流（RBF）进行无创测量。方法研究了名为 AO-LDV（自适应光学激光多普勒速度计）的原型机，它将新型绝对激光多普勒速度计与自适应光学眼底照相机（rtx1，Imagine Eyes®，法国奥赛公司）结合在一起，用于测量视网膜血管直径与绝对 RBF 的函数关系，并同时测量红细胞速度。对健康受试者（15 人）和视网膜静脉闭塞（RVO，6 人）患者的 RBF 进行了测量。我们还评估了两款测量视网膜血管直径的软件：软件 1（自动血管检测、轮廓分析）和软件 2（基于使用深度神经网络对血管进行语义分割，采用 M2u-Net 架构）。结果软件 2 的视网膜血管自动测量率（12320 张 AO 图像中的 99.5%）高于软件 1（64.9%），测量范围更广（75.5 ± 15.7 μm vs 70.9 ± 19.8 μm，p < 0.001）。对于健康受试者（n = 15），测量一只眼睛的所有视网膜静脉以获得总 RBF。健康受试者的总 RBF 为 37.8 ± 6.8 μl/min。视网膜血管直径与最大速度之间存在明显的线性相关（斜率 = 0.1016；p <；0.001；r2 = 0.8597），视网膜血管直径与血流量之间存在明显的功率曲线相关（3.63 × 10-5 × D2.54；p <；0.001；r2 = 0.7287）。总 RBF 与收缩压和舒张压、眼灌注压、心率或血细胞比容之间没有明显关系。对于 RVO 患者（n = 6），与对侧健康眼（11.07 ± 4.53 μl/min）相比，闭塞静脉的 RBF 明显下降（3.51 ± 2.25 μl/min）。对于闭塞血管，直径与速度之间的斜率为 0.0195（p < 0.001；r2 = 0.6068），直径与流量之间的关系为 Q = 9.91 × 10-6 × D2.41（p < 0.01；r2 = 0.2526）。

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来源期刊

Microvascular research 医学-外周血管病

CiteScore

6.00

自引率

3.20%

发文量

158

审稿时长

43 days

期刊介绍： Microvascular Research is dedicated to the dissemination of fundamental information related to the microvascular field. Full-length articles presenting the results of original research and brief communications are featured. Research Areas include: • Angiogenesis • Biochemistry • Bioengineering • Biomathematics • Biophysics • Cancer • Circulatory homeostasis • Comparative physiology • Drug delivery • Neuropharmacology • Microvascular pathology • Rheology • Tissue Engineering.