{"title":"Development of a multiplex real time-PCR assay to estimate the distribution of the genus Silurus in Lake Biwa using environmental DNA","authors":"Tetsuro Inamoto, Yuu Kishimoto, Hisashi Okuyama, Ryoichi Tabata, Koichiro Nakano, Jun-ichi Takahashi","doi":"10.1007/s12686-023-01340-4","DOIUrl":null,"url":null,"abstract":"<p>Three species of catfish, <i>Silurus biwaensis</i>, <i>Silurus lithophilus</i>, and <i>Silurus asotus</i>, are identified in Lake Biwa. Notably, <i>S. biwaensis</i> and <i>S. lithophilus</i> are endemic species in Lake Biwa; however, their detailed distribution and ecology almost remain unknown. In this study, we developed catfish species-specific primer probe sets based on their whole genome. Using these, we analyzed the water samples collected from three tanks where <i>S. biwaensis</i>, <i>S. lithophilus</i>, and <i>S. asotus</i> were experimentally reared, respectively, and established a new detection system after confirming each primer probe set to be effective. Finally, we conducted environmental DNA analysis for catfishes in Lake Biwa using field samples. Overall, our results showed that environmental DNA analysis is a useful monitoring method for confirming the presence of endemic catfishes in Lake Biwa.</p>","PeriodicalId":10625,"journal":{"name":"Conservation Genetics Resources","volume":"20 1","pages":""},"PeriodicalIF":0.9000,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Conservation Genetics Resources","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1007/s12686-023-01340-4","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIODIVERSITY CONSERVATION","Score":null,"Total":0}
引用次数: 0
Abstract
Three species of catfish, Silurus biwaensis, Silurus lithophilus, and Silurus asotus, are identified in Lake Biwa. Notably, S. biwaensis and S. lithophilus are endemic species in Lake Biwa; however, their detailed distribution and ecology almost remain unknown. In this study, we developed catfish species-specific primer probe sets based on their whole genome. Using these, we analyzed the water samples collected from three tanks where S. biwaensis, S. lithophilus, and S. asotus were experimentally reared, respectively, and established a new detection system after confirming each primer probe set to be effective. Finally, we conducted environmental DNA analysis for catfishes in Lake Biwa using field samples. Overall, our results showed that environmental DNA analysis is a useful monitoring method for confirming the presence of endemic catfishes in Lake Biwa.
利用环境 DNA 开发一种多重 real-time-PCR 检测方法，以估算琵琶湖中蚕蛾属的分布情况
在琵琶湖中发现了三种鲶鱼：琵琶鲶（Silurus biwaensis）、石斑鲶（Silurus lithophilus）和鲇鱼（Silurus asotus）。值得注意的是，S. biwaensis 和 S. lithophilus 是琵琶湖的特有物种，但它们的详细分布和生态几乎仍然未知。在本研究中，我们根据鲶鱼的全基因组开发了鲶鱼物种特异性引物探针组。利用这些探针组，我们分析了分别从实验饲养 S. biwaensis、S. lithophilus 和 S. asotus 的三个水箱中采集的水样，并在确认每个引物探针组有效后建立了新的检测系统。最后，我们利用野外样本对琵琶湖中的鲶鱼进行了环境 DNA 分析。总之，我们的结果表明，环境 DNA 分析是确认琵琶湖特有鲶鱼是否存在的有效监测方法。
期刊介绍：
Conservation Genetics Resources promotes the conservation of genetic diversity and advances the study of conservation genetics by providing rapid publication of technical papers and reviews on methodological innovations or improvements, computer programs, and genomic resources, as well as on the practical application of these resources towards the development of effective conservation policy and practice.
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