Microbiological and molecular characterization of E. coli and Salmonella isolated from diarrheic calves

Michael Salama, Waleed Younis, Hams M. A. Mohamed, S. Sultan
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Abstract

Diarrhea is one of the most common fatal disease of neonatal calves regardless of farm welfare. What makes treatment of these cases challenging is the increase in the incidence of multi-drug resistant bacteria, including E. coli and Salmonella. To isolate, identify, molecular characterize of E. coli and salmonella in diarrheic calves, 50 fecal samples were collected and analyzed aseptically. The preliminary result showed that 62% and 14% samples were positive for E. coli and salmonella respectively. Among the E.coli isolates O86:K59, O128:K, O55:K59 (2), O86:K61, O119:K58, O08:K61, O126:K71 serotypes was detected in eight isolates while one isolate was untypeable. Three salmonella serotypes, Salmonella Typhimurium, Salmonella Anatum and Salmonella Florida was detected in the isolated samples . All of the E. coli strains had eaeA genes while 16.7 and 11.1% of them harbored stx1 and stx2 genes respectively. About 71.4% of salmonella isolates were positive for all five pathogenicity island genes from SPI-1 to SPI-5, while SPI-1, SPI-2 and SPI-5 were detected in 14.3% and SPI-2, SPI-3, SPI-4 and SPI-5 were positive in 14.3%. All E. coli strains were resistant to Ampicillin, Amoxicillin/Clavulanic, Cefazolin and Aztreonam., and all Salmonella serovars were resistant to Cefazolin, Chloramphenicol, Gentamycin, Kanamycin and Aztreonam. The present study identified multidrug resistant E. coli and salmonella as the common pathogen of calf diarrhea in the study area and the pathogens harbored common virulence markers of human diarrheic strains which might cause a food borne outbreak in the future.
从腹泻犊牛中分离出的大肠杆菌和沙门氏菌的微生物学和分子鉴定
腹泻是最常见的致命疾病之一的新生牛犊不管农场福利。使这些病例的治疗具有挑战性的是多重耐药细菌的发病率增加,包括大肠杆菌和沙门氏菌。为分离、鉴定腹泻犊牛大肠杆菌和沙门氏菌,对50份犊牛粪便进行无菌分析。初步结果显示,大肠杆菌和沙门氏菌检出率分别为62%和14%。其中O86:K59、O128:K、O55:K59(2)、O86:K61、O119:K58、O08:K61、O126:K71共检出8株血清型,1株无法分型。分离样品中检出鼠伤寒沙门菌、鸭状沙门菌和佛罗里达沙门菌3种血清型。所有大肠杆菌均含有eaeA基因,其中stx1和stx2基因分别占16.7%和11.1%。其中,71.4%的沙门氏菌分离株均检测到SPI-1 ~ SPI-5 5个致病岛基因,14.3%的分离株检测到SPI-1、SPI-2和SPI-5, 14.3%的分离株检测到SPI-2、SPI-3、SPI-4和SPI-5。所有大肠杆菌菌株均对氨苄西林、阿莫西林/克拉维兰、头孢唑林和氨曲南耐药。所有沙门氏菌血清型均对头孢唑林、氯霉素、庆大霉素、卡那霉素和氨曲南耐药。本研究发现,多药耐药大肠杆菌和沙门氏菌是研究区犊牛腹泻的常见致病菌,这些致病菌具有人类腹泻菌株的常见毒力标记,可能在未来引起食源性暴发。
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