Effect of Aspergillus flavus filtrate on different stages of Callosobruchus maculatus (Fabricius.) (Chrysomelidae: Coleoptera) on Broad beans

Abstract

An experiment was conducted to use A.flavus filtrate in treating different stages of C.maculatus (eggs, larvae, pupae) after diluting it with sterile distilled water at concentrations 30%, 50%, 70% directly to calculate the killing rates after (1, 3, 5) days After treatment, the best results of egg inhibition were in the fifth day, with rates reaching 36.9, 53.7 and 83.87%  respectively, and the best results of killing larvae were in the 3rd, 5th, and 5th day, respectively, with rates reaching 50.33, 80.47and 93.93% respectively, and the best results were Killing of the pupa in the 5th, 5th, and 5th day respectively, with rates that reached 63.50, 86.93and 96.97% respectively, and the best results of killing for adults were in the 3rd, 5th, and5th days respectively, with rates that reached 43.73, 90.83, and 93.93% respectively. The best results of killing were in the 1th, 3th, and 5th day respectively on adults of C. maculatus via the indirect method, as the highest killing rates were at the concentration of 70% and reached 56.83, 60.36 and 67.1% respectively, The best results were on the amount of laying eggs from an adult C. maculatus and by direct and indirect spraying on females. The results showed that the lowest rate of laying eggs for one female after three days of mating was zero eggs/female with a concentration of 70% compared to the indirect method which reached to 24.13 eggs/female.