Evaluation of hematological and blood biochemical indices in cultured Nile tilapia (Oreochromis niloticus) as affected by using phage therapy against Pseudomonas aeruginosa

IF 1.9 4区 农林科学 Q1 Veterinary
Nahed A. El-Wafai, Sara A.T. El-Ged, Victor S. Bedrous, Mamdouh A. A. Mousa, A. Shami, K. A. Al Syaad, M. A. Bajaber, Ahmed Ezzat Ahmed, M. Ashkan, A. S. Alwabli, Amani Osman Shakak, Fatimah S. Alqahtani, Eman A. Beyari, B. A. Akl
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引用次数: 0

Abstract

Abstract Pseudomonas spp. causes significant losses in aquaculture, consecutive use of antibiotics, and reveals bacterial resistance; therefore, therapeutic bacteriophages, commonly called phages, are a promising potential alternative to antibiotics in the management of bacterial infections of a wide range of organisms, including cultured fish. The novelty of current work represented in examining the lytic activity of four phages and their combination compared to the antibiotic streptomycin on Pseudomonas aeruginosa-infected Nile tilapia (Oreochromis niloticus) while measuring the hematological and blood biochemical parameters as a response for phage therapy. This study evaluated the in vitro killing curve for each phage using a growth curve that measured the OD600 after a single phage suspension was combined with the host P. aeruginosa, considered the best multiplicity of infection (MOI) for each phage. A144 healthy fish were acclimatized in the laboratory and divided into six groups: control, P. aeruginosa-infected fish, streptomycin, phage Ps1, Ps2, both (Ps1 and Ps2), were added to the T3, T4, T5, and T6 groups, respectively. Our findings demonstrated that P. aeruginosa infection caused surface body hemorrhages, tail and fin rot, irritated skin, superficial ulcers, and 100% mortality through 14 days. P. aeruginosa caused a reduction in hemoglobin (Hb), red blood cells (RBCs), platelet number (PLt), and platelet crit (PCT) count protein, albumin, and A/G ratio; however, an increase in hematocrit (Hct); Red cell distribution width (RDW), PDW, MPV compared to other groups after three days of infection and the effects increased after 12 days post-infection. The fish vaccinated with P1 (T4) and P1+P2 (T6) showed enhanced levels of Hb, RBCs, PLt, PCt, protein, albumin and decreased levels of RDW, PDW, MPV, and liver and kidney enzymes with enhanced contents more than streptomycin and closer to the control group. The biochemical markers recorded significant changes indicating liver and kidney impairments due to the infection with P. aeruginosa. It can concluded that P1 and P1+P2 combinations could use as therapy in Pseudomonas-infectd fish to enhance their blood parameters and performance.
使用噬菌体疗法抗铜绿假单胞菌对养殖尼罗罗非鱼(Oreochromis niloticus)血液学和血液生化指标的影响评估
摘要假单胞菌在水产养殖、连续使用抗生素中造成重大损失,并显示出细菌耐药性;因此,治疗性噬菌体,通常被称为噬菌体,是一种有希望的潜在抗生素替代品,可用于管理包括养殖鱼类在内的多种生物的细菌感染。当前工作的新颖性体现在检查四种噬菌体及其组合对铜绿假单胞菌感染的尼罗罗非鱼(Oreochromis niloticus)的溶解活性,同时测量作为噬菌体治疗反应的血液学和血液生化参数。本研究通过测定单个噬菌体悬浮液与宿主铜绿假单胞菌(P. aeruginosa)结合后OD600的生长曲线来评估每个噬菌体的体外杀伤曲线,认为每个噬菌体的最佳感染多重性(multiplicity of infection, MOI)。在实验室驯化A144条健康鱼,将其分为6组,分别在T3、T4、T5和T6组中添加对照组、铜绿假单胞菌感染鱼、链霉素、噬菌体Ps1和Ps2 (Ps1和Ps2)。我们的研究结果表明,铜绿假单胞菌感染可引起体表出血、尾鳍腐烂、皮肤刺激、浅表溃疡和14天内100%的死亡率。铜绿假单胞菌引起血红蛋白(Hb)、红细胞(rbc)、血小板数量(PLt)和血小板临界值(PCT)计数、蛋白、白蛋白和a /G比的降低;然而,红细胞压积(Hct)增加;感染后3 d红细胞分布宽度(RDW)、PDW、MPV与其他组比较,感染后12 d效果增强。接种P1 (T4)和P1+P2 (T6)的鱼Hb、rbc、PLt、PCt、蛋白、白蛋白水平升高,RDW、PDW、MPV、肝脏和肾脏酶水平降低,其升高的含量高于链霉素,接近对照组。生化指标有明显变化,提示铜绿假单胞菌感染引起肝肾损害。由此可见,P1和P1+P2联合治疗假单胞菌感染的鱼可以改善其血液参数和生产性能。
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来源期刊
Annals of Animal Science
Annals of Animal Science AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
3.80
自引率
5.30%
发文量
138
期刊介绍: Annals of Animal Science accepts original papers and reviews from the different topics of animal science: genetic and farm animal breeding, the biology, physiology and reproduction of animals, animal nutrition and feedstuffs, environment, hygiene and animal production technology, quality of animal origin products, economics and the organization of animal production.
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