Frequency of Vancomycin and Aminoglycoside Resistance Genes in Enterococcus spp. Isolated from Chicken Raw Meat Samples

Davoud Afshar, Reza Shakiba, F. Haghi, H. Zeighami
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Abstract

Background: Enterococci are main normal microbial flora of both humans and animals and can survive in a diverse range of environments. These bacteria carry out aminoglycoside and vancomycin resistance genes and spread them in environment by many routs such as chicken meat products. The present study was aimed to determine the frequency of aminoglycoside and vancomycin resistant genes in Enterococcus species isolated from chicken meat specimens. Methods: A total of 250 chicken raw meat specimens was prepared from slaughterhouses at Zanjan province, cultured at BHI broth and incubated at 37°C for 24h. The positive cultures were sub-cultured in blood agar plates and grown colonies identified using phenotypical and biochemical tests. Antibiotic susceptibility was determined according to the Clinical and Laboratory Standards Institute (CLSI) standards and PCR assays were performed to detect vanA, vanB, aph (2")1c, aph (2")1b, aph (2")1d, ant(3'), aph(3')IIIa, ant(4')1a, ant(6') and aac(6') genes. Results: In total, 100 Enterococcus species isolated from 250 specimens and 35% of them belonged to E. faecalis and the others were E. faecium (65%). The prevalence of the vanA, vanB, aph (2")1c, aph (2")1b, aph (2")1d, ant(3'), aph(3')IIIa, ant(4')1a, ant(6') and aac(6') genes among the 100 Enterococcus species was 14%, 12%, 10%, 1%, 2%, 50%, 26%, 9%, 18% and 22%, respectively. Conclusion: The current study revealed that the rate of antimicrobial resistance genes to aminoglycosides and vancomycin was worrying and health measurements in meat products industries must be performed to prevent spread of antimicrobial resistance elements among bacteria.
鸡肉生肉样本中分离出的肠球菌对万古霉素和氨基糖苷类药物耐药基因的频率
背景:肠球菌是人类和动物的主要正常微生物菌群,可以在多种环境中生存。这些细菌携带氨基糖苷和万古霉素耐药基因,并通过鸡肉制品等多种途径在环境中传播。本研究旨在确定从鸡肉标本中分离的肠球菌中氨基糖苷和万古霉素耐药基因的频率。方法:从赞詹省屠宰场制备250份鸡肉生肉标本,在BHI肉汤中培养,37℃孵育24h。阳性培养物在血琼脂板中继代培养,用表型和生化试验鉴定生长菌落。按照美国临床与实验室标准协会(CLSI)标准进行药敏检测,PCR检测vanA、vanB、aph(2”)1c、aph(2”)1b、aph(2”)1d、ant(3”)、aph(3”)IIIa、ant(4”)1a、ant(6”)和aac(6”)基因。结果:250份标本共分离到100种肠球菌,其中粪肠球菌占35%,其余为粪肠球菌(65%)。在100种肠球菌中,vanA、vanB、aph(2’)1c、aph(2’)1b、aph(2’)1d、ant(3’)、aph(3’)IIIa、ant(4’)1a、ant(6’)和aac(6’)基因的阳性率分别为14%、12%、10%、1%、2%、50%、26%、9%、18%和22%。结论:目前的研究表明,对氨基糖苷类和万古霉素的耐药基因的比例令人担忧,肉制品行业必须采取卫生措施,防止耐药元素在细菌中传播。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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