Bioremediation of heavy metal polluted soil using plant growth promoting bacteria: an assessment of response

IF 1.9 4区 环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES
Ifeoma Anthonia Okpara-Elom, Charles Chike Onochie, Michael Okpara Elom, Emmanuel Ezaka, Ogbonnaya Elom
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Abstract

Soil samples were collected from research farm of Ebonyi State University and mining site in Izzi Local Government Area (L.G.A) of Ebonyi State. Rhizobacteria from rhizosphere of maize were identified using molecular methods. Tolerance of the isolates to heavy metals was assessed using standard laboratory techniques. The heavy metal profile of the soil was determined using atomic absorption spectrophotometer (AAS). Ability of the PGPB to remediate heavy metal polluted soil was assessed with hot plate aqua-regiae digestion method. The isolates were identified as: Pseudomonas fluorescens strain 5113, Alcaligenes faecalis strain P156, Pseudomonas syringae HS191, Bacillus subtilis strain NBSL51 and Bacillus cereus strain 20 UPMNR. P. fluorescens exhibited maximum tolerance at different concentrations of metals while A. faecalis had minimum tolerance. The heavy metal profile of the soil was determined and expressed in ppm as lead (25.0), copper (2.4), chromium (1.2) and zinc (16.0). The Isolates remediated the heavy metals. P. fluorescens + B. cereus maximally degraded the metals with values of (Pb=87.2%, Zn=69.25%, Cr =67.5%) while B. cereus alone degraded 70.0% Cu. P. fluorescens + B. cereus is recommended for soil that is contaminated with chromium, lead and zinc while B. cereus is recommended for copper contaminated soils.

植物促生菌对重金属污染土壤的生物修复效果评价
摘要土壤样品采集于鄂邦伊州伊兹地方政府区鄂邦伊州鄂邦伊州大学研究农场和矿区。采用分子方法对玉米根际根际细菌进行了鉴定。采用标准实验室技术评估了分离株对重金属的耐受性。采用原子吸收分光光度计(AAS)测定了土壤中重金属的含量。采用热板法评价了PGPB对重金属污染土壤的修复能力。分离菌株鉴定为:荧光假单胞菌5113、粪碱菌P156、丁香假单胞菌HS191、枯草芽孢杆菌NBSL51和蜡样芽孢杆菌20upmnr。荧光假单胞菌对不同浓度金属的耐受性最大,粪单胞菌对不同浓度金属的耐受性最小。测定了土壤重金属剖面,以ppm表示为铅(25.0)、铜(2.4)、铬(1.2)和锌(16.0)。隔离物修复了重金属。荧光假单胞菌+蜡样芽孢杆菌对金属的降解率最高,Pb = 87.2%, Zn = 69.25%, Cr =67.5%,而蜡样芽孢杆菌对铜的降解率为70.0%。建议在铬、铅和锌污染的土壤中使用荧光假单胞菌+蜡样芽孢杆菌,而在铜污染的土壤中推荐使用蜡样芽孢杆菌。
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来源期刊
Bioremediation Journal
Bioremediation Journal ENVIRONMENTAL SCIENCES-
CiteScore
5.30
自引率
0.00%
发文量
36
审稿时长
9 months
期刊介绍: Bioremediation Journal is a peer-reviewed quarterly that publishes current, original laboratory and field research in bioremediation, the use of biological and supporting physical treatments to treat contaminated soil and groundwater. The journal rapidly disseminates new information on emerging and maturing bioremediation technologies and integrates scientific research and engineering practices. The authors, editors, and readers are scientists, field engineers, site remediation managers, and regulatory experts from the academic, industrial, and government sectors worldwide. High-quality, original articles make up the primary content. Other contributions are technical notes, short communications, and occasional invited review articles.
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