Pollen Dispersion is a Key Factor for Autonomous Fruit Set Under High Temperatures in the Capsicum annuum ‘Takanotsume’

Abstract

Chili pepper is cultivated in the absence of fruit-promoting treatments or insect pollinators. The fertilization ability of both males and females, as well as natural self-pollination ability under high temperatures, are necessary for autonomous set fruit under high temperatures. The reproductive traits related to autonomous self-pollination ability and fertilization ability of both males and females after pollination under control (CK) and high temperature (HT) treatments were investigated in this study. ‘Takanotsume’ (TK) showed a significantly higher percentage of autonomous fruit set than ‘Peruvian Purple’ (PP) in both CK and HT treatments, suggesting that TK has a strong autonomous fruit set regardless of temperature. On the other hand, the percentage of autonomous fruit set of PP was 0% in the HT treatment, while autonomous fruit set was observed in the CK treatment. Therefore, TK had autonomous fruit set ability even at high temperatures when PP did not have any. TK had more pollen on the stigma than PP, suggesting that TK has a higher ability for autonomous self-pollination. Flower morphology was not considered a factor in the autonomous self-pollination of TK. In contrast, TK tended to release more pollen than PP. One factor that can support autonomous self-pollination is pollen dispersion. A significant difference was observed in the fruit set rate during artificial pollination between the treatments (CK and HT) of the pollen parent and the cultivars (TK and PP) of the seed parent. In fact, female fertility in TK was higher than that in PP, regardless of the temperature condition. Almost no fruit set of PP was observed via autonomous self-pollination in the HT treatment, but fruits were set by artificial pollination using both the pollen and pistil of PP in the HT treatment. This result is consistent with the finding that PP exhibits decreased autonomous self-pollination at high temperatures. The higher autonomous fruit-set ability of TK than PP under high temperatures may be attributed not only to superior female fertility, but also to its autonomous self-pollination ability under high temperatures. Therefore, pollen dispersal ability under high temperatures was considered a key factor for autonomous fruit-set.