{"title":"Identification and Characterisation of a Novel Phosphatidylinositol-Specific Phospholipase C Having Implications in the Pathogenesis of Leptospirosis","authors":"T. H. Samaha","doi":"10.3103/s0096392522020092","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Tracing secreted proteins from the culture supernatant enabled the identification of a novel phosphatidylinositol-specific phospholipase C of <i>Leptospira</i>. Topology predictions established the extracellular localisation nature. Functional annotation designated the protein as an exotoxin and an antigen with virulent factor potential. Besides, immunoblotting analysis of the culture supernatant with the leptospirosis positive serum samples confirmed its expression and exposure to the immune system. In addition, conserved expression of the gene among the representatives of pathogenic, intermediates, and non-pathogenic <i>Leptospira</i> were uncovered and signified the potential as a vaccine candidate. Hence, the data highlights the characteristics of a novel phosphatidylinositol-specific phospholipase C of <i>Leptospira</i> with possible implications in pathogenesis.</p>","PeriodicalId":19004,"journal":{"name":"Moscow University Biological Sciences Bulletin","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Moscow University Biological Sciences Bulletin","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3103/s0096392522020092","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0
Abstract
Tracing secreted proteins from the culture supernatant enabled the identification of a novel phosphatidylinositol-specific phospholipase C of Leptospira. Topology predictions established the extracellular localisation nature. Functional annotation designated the protein as an exotoxin and an antigen with virulent factor potential. Besides, immunoblotting analysis of the culture supernatant with the leptospirosis positive serum samples confirmed its expression and exposure to the immune system. In addition, conserved expression of the gene among the representatives of pathogenic, intermediates, and non-pathogenic Leptospira were uncovered and signified the potential as a vaccine candidate. Hence, the data highlights the characteristics of a novel phosphatidylinositol-specific phospholipase C of Leptospira with possible implications in pathogenesis.
期刊介绍：
Moscow University Biological Sciences Bulletin is forum for research in all important areas of modern biology. It publishes original work on qualitative, analytical and experimental aspects of research. The scope of articles to be considered includes plant biology, zoology, ecology, evolutionary biology, biophysics, genetics, genomics, proteomics, molecular biology, cell biology, biochemistry, endocrinology, immunology, physiology, pharmacology, neuroscience, gerontology, developmental biology, bioinformatics, bioengineering, virology, and microbiology.
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