Detection and characterization of zoonotic Bartonella spp. in rodents and shrews ectoparasites from Kigoma and Morogoro regions, Tanzania

IF 0.8 4区生物学 Q3 ZOOLOGY

Mammalia Pub Date : 2023-12-04 DOI:10.1515/mammalia-2023-0072

Ginethon G. Mhamphi, Venance T. Msoffe, Charles M. Lyimo, Abdul S. Katakweba, Apia W. Massawe, Erick V. G. Komba, Ladslaus L. Mnyone

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Abstract

Bartonellosis is a vector-borne disease which is increasingly threatening the health of humans and animals worldwide consequent to the growing wildlife-animals-human interactions. Little is known about the epidemiology of this disease in Tanzania. In this study we investigated and characterized Bartonella species in small mammals’ ectoparasites from potentially high-risk areas in the country. A total of 141 ectoparasites pools of mites, fleas, ticks, and lice were analyzed using conventional PCR and sequencing. Bartonella DNA was detected in 34.8 % of the tested ectoparasite pools, with mites at 32.9 %, fleas at 40 %, ticks at 12.5 %, and lice at 50 %. Phylogenetic analysis showed that the Bartonella spp. genotypes were closely related to those found in Uganda, Kenya, and South Africa. Different genotypes with independent haplotypes were observed, although most Bartonella spp. from fleas shared the same haplogroup. The confirmed presence of Bartonella elizabethae and Bartonella tribocorum in field and house rodents emphasizes the prevailing transmission risk of zoonotic infections in the study areas and beyond. Screening of humans, companion animals, and livestock in potentially high-risk areas in Tanzania is necessary in order to inform the development of responsive surveillance and control strategies.

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坦桑尼亚基戈马和莫罗戈罗地区鼠类和鼩鼱中人畜共患巴尔通体外寄生虫的检测和鉴定

巴尔通体病是一种媒介传播的疾病，随着野生动物-动物-人类相互作用的增加，它正日益威胁着全世界人类和动物的健康。人们对这种疾病在坦桑尼亚的流行病学知之甚少。在本研究中，我们调查并鉴定了来自国内潜在高危地区的小型哺乳动物体表寄生虫中的巴尔通体种类。采用常规PCR和测序方法对141个螨、蚤、蜱、虱体外寄生虫池进行分析。体外寄生虫库中巴尔通体DNA检出率为34.8%，螨为32.9%，蚤为40%，蜱为12.5%，虱为50%。系统发育分析表明，该巴尔通体基因型与在乌干达、肯尼亚和南非发现的巴尔通体基因型密切相关。蚤类巴尔通体具有相同的单倍群，但不同的基因型具有独立的单倍型。野外和室内啮齿类动物中已证实存在伊丽莎白巴尔通体和摩擦体巴尔通体，这强调了在研究区域内外人畜共患感染的普遍传播风险。有必要对坦桑尼亚潜在高风险地区的人类、伴侣动物和牲畜进行筛查，以便为制定反应性监测和控制战略提供信息。

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来源期刊

Mammalia 生物-动物学

CiteScore

2.20

自引率

10.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Mammalia is an international, multidisciplinary, bimonthly journal devoted to the inventory, analysis and interpretation of mammalian diversity. It publishes original results on all aspects of the systematics and biology of mammals with a strong focus on ecology, including biodiversity analyses, distribution habitats, diet, predator-prey relationships, competition, community analyses and conservation of mammals. The journal also accepts submissions on sub-fossil or recently extinct mammals.