BdASR5 Positively Regulates Drought Tolerance by Mediating ABA Signaling Pathway in Brachypodium distachyon

IF 2.2 4区生物学 Q2 PLANT SCIENCES

Journal of Plant Biology Pub Date : 2023-11-17 DOI:10.1007/s12374-023-09411-7

Jin Seok Yoon, Depika Prasad, Yong Weon Seo

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引用次数: 0

Abstract

Abscisic acid-, stress-, and ripening-induced (ASR) proteins are involved in response to abiotic stresses. However, the precise role of Brachypodium ASR genes in enhancing tolerance under drought stress conditions remains to be determined. In this study, we characterized the BdASR5 gene and determined that it has a function in drought stress tolerance. Overexpression of BdASR5 confers tolerance to drought stress in Brachypodium. Overexpression of BdASR5 resulted in higher relative water content and chlorophyll content, and lower ion leakage than WT plants under drought stress conditions. Moreover, overexpression of BdASR5 displayed increased antioxidant enzyme activity and upregulated expression of ROS-related, stress-related, and ABA-dependent pathway genes under drought stress condition. In addition, overexpression of BdASR5 showed hypersensitive to exogenous ABA at the germination stage. Moreover, overexpression of BdASR5 showed increased stomatal closure and decreased stomata conductance under ABA conditions. Collectively, these results suggest that BdASR5 functions as a positive regulator in response to drought stress.

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BdASR5通过介导ABA信号通路正向调控短柄草的抗旱性

脱落酸、胁迫和成熟诱导(ASR)蛋白参与对非生物胁迫的反应。然而，短茅ASR基因在干旱胁迫条件下增强耐受性中的确切作用仍有待确定。在这项研究中，我们鉴定了BdASR5基因，并确定了它在干旱胁迫耐受中具有功能。BdASR5的过表达使短柄植物对干旱胁迫具有耐受性。在干旱胁迫条件下，BdASR5过表达导致植株相对含水量和叶绿素含量高于WT植株，离子泄漏量低于WT植株。此外，干旱胁迫下，BdASR5的过表达增加了抗氧化酶活性，并上调了ros相关、胁迫相关和aba依赖通路基因的表达。此外，BdASR5的过表达在萌发期表现出对外源ABA的超敏感。此外，在ABA条件下，BdASR5的过表达增加了气孔关闭，降低了气孔导度。综上所述，这些结果表明BdASR5在干旱胁迫下起着积极的调节作用。

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来源期刊

Journal of Plant Biology 生物-植物科学

CiteScore

5.40

自引率

3.40%

发文量

审稿时长

1 months

期刊介绍： Journal of Plant Biology, an official publication of the Botanical Society of Korea, is an international journal devoted to basic researches in biochemistry, cellular biology, development, ecology, genetics, molecular biology, physiology, and systematics of plants. The Journal publishes the following categories of paper: Original articles -- For publication in Journal of Plant Biology the manuscript must provide a significant new contribution to our understanding of plants. All areas of plant biology are welcome. No limit on the length, but a concise presentation is encouraged. Reviews -- Invited by the EiC. Brief Communications -- Concise but independent report representing significant contribution to plant science. The Botanical Society of Korea was founded on November 30, 1957 to promote studies, disseminate and exchange information on the field of plant biology. The first issue of The Korean Journal of Botany, the official publication of the society, was published on April 1, 1958. It was published twice a year, but quarterly from 5th volume in 1962. In 1994, it was renamed to Journal of Plant Biology and published in English since 1996. The journal entered its 50th year of publication in 2007.