Dual culture of the oomycete Lagenisma coscinodisci Drebes and Coscinodiscus diatoms as a model for plankton/parasite interactions

4区地球科学 Q2 Agricultural and Biological Sciences

Helgoland Marine Research Pub Date : 2019-04-16 DOI:10.1186/s10152-019-0523-0

Anthony Buaya, Alexandra Kraberg, Marco Thines

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引用次数: 22

Abstract

Diatoms are thought to provide about 40% of total global photosynthesis and diatoms of the genus Coscinodiscus are an important, sometimes dominant, cosmopolitan component of the marine diatom community. The oomycete parasitoid Lagenisma coscinodisci is widespread in the northern hemisphere on its hosts in the genus Coscinodiscus. Because of its potential ecological importance, it would be a suitable pathogen model to investigate plankton/parasite interactions, but the species cannot be cultivated on media without its host, so far. Thus, it was the aim of this study to explore the potential of dual culture of host and pathogen in the laboratory and to optimise cultivation to ensure a long-term cultivation of the pathogen. Here, we report successful cultivation of a single spore strain of L. coscinodisci (Isla), on several Coscinodiscus species and strains, as well as the establishment of a cultivation routine with Coscinodiscus granii (CGS1 and CG36), which enabled us to maintain the single spore strain for more than 3 years in 6 cm Petri dishes and 10 ml tissue culture flasks. This opens up the opportunity to study the processes and mechanism in plankton/parasitoid interactions under controlled conditions.

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作为浮游生物/寄生虫相互作用模型的卵菌尾盘藻和尾盘硅藻的双重培养

硅藻被认为提供了全球总光合作用的约40%，而尾盘藻属硅藻是海洋硅藻群落中重要的，有时占主导地位的世界性组成部分。寄生卵菌尾盘Lagenisma coscinodisci广泛分布于北半球的尾盘属宿主上。由于其潜在的生态重要性，它将是一个合适的病原体模型来研究浮游生物与寄生虫的相互作用，但到目前为止，该物种还不能在没有宿主的培养基上培养。因此，本研究的目的是探索寄主和病原体在实验室双重培养的潜力，并优化培养以确保病原体的长期培养。本研究成功培养了一株尾盘菌单孢子菌株(Isla)，并与大尾盘菌(CGS1和CG36)建立了培养常规，使单孢子菌株在6 cm的培养皿和10 ml的组织培养瓶中保持了3年以上。这为在受控条件下研究浮游生物/拟寄生虫相互作用的过程和机制提供了机会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Helgoland Marine Research 地学-海洋学

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Helgoland Marine Research is an open access, peer reviewed journal, publishing original research as well as reviews on all aspects of marine and brackish water ecosystems, with a focus on how organisms survive in, and interact with, their environment. The aim of Helgoland Marine Research is to publish work with a regional focus, but with clear global implications, or vice versa; research with global emphasis and regional ramifications. We are particularly interested in contributions that further our general understanding of how marine ecosystems work, and that concentrate on species’ interactions.