Clonal selection of wine yeasts with differential adsorption activities towards phenolics and ochratoxin A

IF 1.8 4区 农林科学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Andrea Caridi, Rossana Sidari, Andrea Pulvirenti, Giuseppe Blaiotta, Alberto Ritieni
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引用次数: 0

Abstract

ABSTRACT

To design a rapid, simple, and low-cost procedure for yeast selection with differential adsorption activities toward phenolics and ochratoxin A, 284 yeast strains were screened. This was done by evaluating the type of growth during grape must fermentation, acetic acid production on Chalk agar, H2S production on BiGGY agar, and spore-formation on acetate agar. After that initial step, 84 strains were pre-selected and further studied by Petri plate tests and to determine their wine-making ability in trials and evaluating their differential adsorption activities toward phenolics and ochratoxin A. Three yeast strains were selected based on the above evaluations. After confirming that they belonged to Saccharomyces cerevisiae species and were diploids, a spore clonal selection was performed. The strain Sc1741A_1D was selected and used in winemaking at six Calabrian wineries and found to be suitable as wine starter to improve quality and safety of red wines.

对酚类物质和赭曲霉毒素A具有不同吸附活性的酿酒酵母克隆选择
摘要为了设计一种快速、简单、低成本的对酚类物质和赭曲霉毒素a具有不同吸附活性的酵母菌筛选方法,筛选了284株酵母菌。这是通过评估葡萄must发酵期间的生长类型,粉笔琼脂上的乙酸产量,BiGGY琼脂上的H2S产量和醋酸琼脂上的孢子形成来完成的。在初步筛选之后,我们对84株酵母菌进行了预筛选,并通过培养皿试验进一步研究了它们的酿酒能力,并评估了它们对酚类物质和赭曲霉毒素a的差异吸附活性。在确定它们属于酿酒酵母菌属(Saccharomyces cerevisiae)属二倍体后,进行了孢子克隆选择。选用Sc1741A_1D菌株,在卡拉布里亚的6家酿酒厂进行了酿酒试验,发现该菌株适合作为葡萄酒的发酵剂,可以提高红葡萄酒的质量和安全性。
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来源期刊
Food Biotechnology
Food Biotechnology 工程技术-生物工程与应用微生物
CiteScore
3.80
自引率
0.00%
发文量
15
审稿时长
>12 weeks
期刊介绍: Food Biotechnology is an international, peer-reviewed journal that is focused on current and emerging developments and applications of modern genetics, enzymatic, metabolic and systems-based biochemical processes in food and food-related biological systems. The goal is to help produce and improve foods, food ingredients, and functional foods at the processing stage and beyond agricultural production. Other areas of strong interest are microbial and fermentation-based metabolic processing to improve foods, food microbiomes for health, metabolic basis for food ingredients with health benefits, molecular and metabolic approaches to functional foods, and biochemical processes for food waste remediation. In addition, articles addressing the topics of modern molecular, metabolic and biochemical approaches to improving food safety and quality are also published. Researchers in agriculture, food science and nutrition, including food and biotechnology consultants around the world will benefit from the research published in Food Biotechnology. The published research and reviews can be utilized to further educational and research programs and may also be applied to food quality and value added processing challenges, which are continuously evolving and expanding based upon the peer reviewed research conducted and published in the journal.
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