Short-term Administration of Naringin Improves Renal Function in Renal Ischemia-reperfusion by Increasing Aquaporin-1 and Aquaporin-2 Levels

IF 1.2 4区医学 Q4 CHEMISTRY, MEDICINAL

Letters in Drug Design & Discovery Pub Date : 2023-11-29 DOI:10.2174/0115701808271000231120094951

Zubeyde Demir, Gozde Acar, Dervis Dasdelen, Rasim Mogulkoc, Abdulkerim Kasim Baltaci

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引用次数: 0

Abstract

Background:: Since renal ischemia-reperfusion (I/R) can lead to a serious health problem, aquaporins have important roles in preventing negative changes in electrolyte-water balance. This study aimed to determine the effect of naringin treatment on renal function and AQP1 and AQP2 levels in the kidney cortex and medulla tissues in experimental renal I/R in rats. Method and Material: The study was carried out on 40 male Wistar-type rats, 8-12 weeks old. Experimental groups were formed as follows: 1) Control, 2) Sham+vehicle, 3) Renal (I/R)+vehicle, 4) Renal I/R+ Naringin (50mg/kg/day) (3 days of administration), and 5) Renal I/R+ Naringin( 100mg/kg/day) (3 days supplementation) group. First, the left kidney was removed by nephrectomy under general anesthesia, and then the right kidney was subjected to 45 minutes of ischemia and then 72 hours of reperfusion. Naringin was given to the experimental animals by an intraperitoneal route at the beginning of the reperfusion, after 24 and 48 hours. At the end of the experiments, first of all, blood samples were taken from the heart in animals under general anesthesia, and then the animals were killed by cervical dislocation, and kidney tissue samples were taken. Osmolarity in plasma and urine and plasma creatinine levels were evaluated. AQP1 and AQP2 levels were analyzed in the kidney cortex and medulla tissues by ELISA and PCR methods. Result:: In kidney tissues, I/R led to a decrease in plasma and urinary osmolality, AQP1 and AQP2 levels in the cortex and medulla, and an increase in urea and creatinine levels (p < 0.001). However, naringin supplementation corrected the deterioration to a certain extent. Conclusion:: The results of the study show that naringin supplementation at different doses, such as 50 or 100 mg/kg, may have protective effects on the deterioration of renal function caused by unilateral nephrectomy and I/R in rats.

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短期给药柚皮苷可通过提高水通道蛋白-1和水通道蛋白-2水平改善肾缺血再灌注时的肾功能

背景:由于肾缺血-再灌注(I/R)可导致严重的健康问题，水通道蛋白在防止电解质-水平衡的负面变化方面具有重要作用。本研究旨在探讨柚皮苷对实验性肾I/R大鼠肾功能及肾皮质、髓质组织AQP1、AQP2水平的影响。方法与材料:选取雄性wistar型大鼠40只，8-12周龄。试验组分为:1)对照组，2)假药+对照剂，3)肾(I/R)+对照剂，4)肾I/R+柚皮苷(50mg/kg/d)(给药3 d)， 5)肾I/R+柚皮苷(100mg/kg/d)(补充3 d)组。先在全身麻醉下切除左肾，右肾缺血45分钟，再灌注72小时。实验动物在再灌注开始时、24小时和48小时后分别腹腔注射柚皮苷。实验结束时，首先在全身麻醉下取动物心脏血样，然后颈椎脱臼处死动物，取肾脏组织样本。测定血浆、尿液渗透压和血浆肌酐水平。采用ELISA和PCR方法分析肾皮质和肾髓质组织AQP1和AQP2水平。结果:在肾组织中，I/R导致血浆和尿渗透压降低，皮质和髓质中AQP1和AQP2水平降低，尿素和肌酐水平升高(p <0.001)。然而，柚皮苷的补充在一定程度上纠正了这种恶化。结论:本研究结果表明，不同剂量的柚皮苷补充，如50或100 mg/kg，可能对单侧肾切除术和I/R引起的大鼠肾功能恶化有保护作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Letters in Drug Design & Discovery 医学-医药化学

CiteScore

1.80

自引率

10.00%

发文量

245

审稿时长

3 months

期刊介绍： Aims & Scope Letters in Drug Design & Discovery publishes letters, mini-reviews, highlights and guest edited thematic issues in all areas of rational drug design and discovery including medicinal chemistry, in-silico drug design, combinatorial chemistry, high-throughput screening, drug targets, and structure-activity relationships. The emphasis is on publishing quality papers very rapidly by taking full advantage of latest Internet technology for both submission and review of manuscripts. The online journal is an essential reading to all pharmaceutical scientists involved in research in drug design and discovery.