Qing-Lang Song, Fang-Hong Nie, Di-Hua Zhu, Yun-Li Wei, Min Zhang, Yao Hu, Wan Wei, Dan-Ju Kang, Zhi-Bao Chen, Hong-Ying Lin, Jin-Jun Chen
{"title":"Identification of PCBs Degrading Bacterial Strains in Zhanjiang Mangrove Sediments","authors":"Qing-Lang Song, Fang-Hong Nie, Di-Hua Zhu, Yun-Li Wei, Min Zhang, Yao Hu, Wan Wei, Dan-Ju Kang, Zhi-Bao Chen, Hong-Ying Lin, Jin-Jun Chen","doi":"10.1134/s1063074023050103","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The strategies to protect the vulnerable Zhanjiang Mangrove Reserve from polychlorinated biphenyls (PCBs) pollution have attracted great attention. PCBs are hard to degrade in the ecosystem and easy to accumulate in sediments. PCBs can be enriched into animals and humans through the food chain, causing “three toxicities”, thus endangering the health of the body. In this study, the PCBs-degrading bacteria in the mangrove sediments of Gaoqiao, Zhanjiang were filtered. 16S rRNA was amplified from the genomes of these bacteria, and the products were purified and sequenced for isolation and identification of the bacteria. A total of 20 strains of PCBs-degrading bacteria were isolated from sediment samples of the low, middle and high tide zones of the Zhanjiang Mangrove Reserve, among which <i>Bacillus</i> and <i>Pandoraea</i> showed the best degradability with a higher level of PCB degradation rates of 92.2 and 97.8%, respectively. These results were helpful for future research, including genetic screening, cloning and restructuring, and instructive for the cultivation of PCBs-degrading bacteria.</p>","PeriodicalId":49584,"journal":{"name":"Russian Journal of Marine Biology","volume":null,"pages":null},"PeriodicalIF":0.5000,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Russian Journal of Marine Biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s1063074023050103","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MARINE & FRESHWATER BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The strategies to protect the vulnerable Zhanjiang Mangrove Reserve from polychlorinated biphenyls (PCBs) pollution have attracted great attention. PCBs are hard to degrade in the ecosystem and easy to accumulate in sediments. PCBs can be enriched into animals and humans through the food chain, causing “three toxicities”, thus endangering the health of the body. In this study, the PCBs-degrading bacteria in the mangrove sediments of Gaoqiao, Zhanjiang were filtered. 16S rRNA was amplified from the genomes of these bacteria, and the products were purified and sequenced for isolation and identification of the bacteria. A total of 20 strains of PCBs-degrading bacteria were isolated from sediment samples of the low, middle and high tide zones of the Zhanjiang Mangrove Reserve, among which Bacillus and Pandoraea showed the best degradability with a higher level of PCB degradation rates of 92.2 and 97.8%, respectively. These results were helpful for future research, including genetic screening, cloning and restructuring, and instructive for the cultivation of PCBs-degrading bacteria.
期刊介绍:
The Russian Journal of Marine Biology was founded in 1975 by Alexey V. Zhirmunsky, member of the Russian Academy of Sciences. The Russian Journal of Marine Biology covers a wide range of research and some applied aspects of marine biology as a synthetic science related to various fields of study on marine biota and environment. It presents fundamental research on biological processes at molecular, cellular, organismal, and populational levels in marine organisms. Consideration is given to marine objects as models in life sciences. The journal also publishes papers dedicated to events in Russian and international marine biological science and the history of biology.