Development and validation of UV–Vis spectrophotometric method for determination of rivastigmine in PBS and biological matrices: Application to ex vivo permeation profiles and in vivo studies from trilayer dissolving microneedle

IF 2.218 Q2 Chemistry

Chemical Data Collections Pub Date : 2023-12-04 DOI:10.1016/j.cdc.2023.101106

Aprilia Paramitha Dwi Putri , Nur Rezky Aulia Ilyas , Dian Arnita Putri Abdullah , Febriansyah Anandha Pratama , Kanaya Shafi Azzahra , Andi Dian Permana

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引用次数: 0

Abstract

Alzheimer's disease (AD) is a neurological illness. AD is often treated with rivastigmine (RV). The present study developed trilayer dissolving microneedle (TDMN) RV delivery system to overcome challenges in RV conventional forms. To enable the assessment of TDMN by ex vivo and in vivo methods, we developed a UV–Visible spectrophotometric method to quantify RV in four media using cobalt thiocyanate as a derivatization reagent. Guidelines set by the International Conference on Harmonization (ICH) were used to validate this approach. Results indicate a correlation coefficient of 0.9999 for all media, with the lower limit of quantification values for Phosphate Buffer Saline, rats’ plasma, skin, and brain matrices were 1.29, 0.67, 0.67, and 0.89 µg/mL, respectively. Significantly, the method is precise and accurate. Finally, to support this study's objective, TDMN formulation was developed, and direct application of the proposed method for quantifying RV in ex vivo and in vivo studies was conducted.

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开发并验证紫外可见分光光度法测定 PBS 和生物基质中的利伐斯的明：将三层溶解微针应用于体内外渗透图谱和体内研究

阿尔茨海默病（AD）是一种神经系统疾病。通常使用利伐斯的明（RV）治疗阿尔茨海默病。本研究开发了三层溶解微针（TDMN）RV 给药系统，以克服 RV 传统形式所面临的挑战。为了能够通过体内外方法评估 TDMN，我们开发了一种紫外-可见分光光度法，使用硫氰酸钴作为衍生试剂对四种介质中的 RV 进行定量。国际协调会议（ICH）制定的准则被用来验证这种方法。结果表明，所有介质的相关系数均为 0.9999，磷酸盐缓冲盐水、大鼠血浆、皮肤和脑基质的定量下限值分别为 1.29、0.67、0.67 和 0.89 µg/mL。值得注意的是，该方法既精确又准确。最后，为了支持本研究的目标，我们开发了 TDMN 配方，并将所提出的方法直接应用于体内外 RV 定量研究。

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来源期刊

Chemical Data Collections Chemistry-Chemistry (all)

CiteScore

6.10

自引率

0.00%

发文量

169

审稿时长

24 days

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