The crystal structure of the human smacovirus 1 Rep domain.

IF 1.1 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Acta crystallographica. Section F, Structural biology communications Pub Date : 2023-12-01 Epub Date: 2023-12-05 DOI:10.1107/S2053230X23009536

Lidia K Limón, Ke Shi, Amy Dao, Jacob Rugloski, Kassidy J Tompkins, Hideki Aihara, Wendy R Gordon, Robert L Evans

引用次数: 0

Abstract

Replication initiator proteins (Reps) from the HUH endonuclease family process specific single-stranded DNA sequences to initiate rolling-circle replication in viruses. Here, the first crystal structure of the apo state of a Rep domain from the smacovirus family is reported. The structure of the human smacovirus 1 Rep domain was obtained at 1.33 Å resolution and represents an expansion of the HUH endonuclease superfamily, allowing greater diversity in bioconjugation-tag applications.

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人类 smacovirus 1 Rep 结构域的晶体结构。

HUH 内切酶家族的复制启动蛋白（Reps）处理特定的单链 DNA 序列，启动病毒中的滚圆复制。本文首次报道了烟曲霉病毒科 Rep 结构域 apo 状态的晶体结构。人类 smacovirus 1 Rep 结构域的结构分辨率为 1.33 Å，代表了 HUH 内切酶超家族的扩展，使生物标记应用更加多样化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta crystallographica. Section F, Structural biology communications BIOCHEMICAL RESEARCH METHODSBIOCHEMISTRY &-BIOCHEMISTRY & MOLECULAR BIOLOGY

CiteScore

1.90

自引率

0.00%

发文量

期刊介绍： Acta Crystallographica Section F is a rapid structural biology communications journal. Articles on any aspect of structural biology, including structures determined using high-throughput methods or from iterative studies such as those used in the pharmaceutical industry, are welcomed by the journal. The journal offers the option of open access, and all communications benefit from unlimited free use of colour illustrations and no page charges. Authors are encouraged to submit multimedia content for publication with their articles. Acta Cryst. F has a dedicated online tool called publBio that is designed to make the preparation and submission of articles easier for authors.