The Impact of Blue Light Irradiation on Keratinocytes in Vitro.

Acta dermatovenerologica Croatica : ADC Pub Date : 2023-11-01

Markus Denzinger, Andreas K Nüssler, Fabian Medved, Sabrina Krauß, Adrien Daigeler, Manuel Held, Claudius Illg

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Abstract

Background: This study examined the effects of irradiation with blue light on HaCaT keratinocytes. As irradiation with blue light is known to be antimicrobial, it offers a promising alternative therapy for contaminated wounds. There is evidence that red light promotes wound healing, but the potential benefits of irradiation with blue light have not yet been adequately investigated.

Methods: The rate of wound closure in sterile and contaminated cells was measured using an in vitro scratch assay wound-healing model. Additionally, cell viability after treatment was determined using a Sulforhodamine B (SRB) assay.

Results: In both the sterile and contaminated groups, treated cells showed delayed wound closure when compared with cells not irradiated with blue light. Additionally, treatment with blue light resulted in poorer viability in the treatment groups.

Conclusion: Although irradiation with blue light may offer a promising alternative therapy for reducing bacterial colonization, our data indicate that re-epithelization may be negatively influenced by blue light. Further research is needed to clarify possible wound healing applications.

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蓝光照射对体外角质形成细胞的影响。

背景:本研究考察了蓝光照射对HaCaT角质形成细胞的影响。由于已知蓝光照射具有抗菌作用，它为污染伤口提供了一种很有前途的替代疗法。有证据表明红光能促进伤口愈合，但蓝光照射的潜在益处尚未得到充分研究。方法:采用体外划伤实验创面愈合模型，测定无菌和污染细胞创面愈合率。此外，使用Sulforhodamine B (SRB)测定处理后的细胞活力。结果:在无菌组和污染组中，与未照射蓝光的细胞相比，处理过的细胞伤口愈合延迟。此外，蓝光治疗导致治疗组的生存能力较差。结论:虽然蓝光照射可能是减少细菌定植的一种有希望的替代疗法，但我们的数据表明蓝光可能会对再上皮产生负面影响。需要进一步的研究来阐明可能的伤口愈合应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Acta dermatovenerologica Croatica : ADC

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