Hybrid bacillus endo-(1-3,1-4)-beta-glucanases: construction of recombinant genes and molecular properties of the gene products.

R Borriss, O Olsen, K K Thomsen, D von Wettstein
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引用次数: 49

Abstract

Hybrid beta-glucanase genes were constructed by the reciprocal exchange of the two halves of the isolated beta-glucanase genes from Bacillus amyloliquefaciens and B. macerans. The beta-glucanase hybrid enzyme 1 (H1) contains the 107 amino-terminal residues of mature B. amyloliquefaciens beta-glucanase and the 107 carboxyl-terminal amino acid residues of B. macerans beta-glucanase. The reciprocal beta-glucanase hybrid enzyme 2 (H2) consists of the 105 amino-terminal residues from the B. macerans enzyme and the carboxyl-terminal 107 amino acids from B. amyloliquefaciens. The biochemical properties of the two hybrid enzymes differ significantly from each other as well as from both parental beta-glucanases. Hybrid beta-glucanase H1 exhibits increased thermostability in comparison to other beta-glucanases, especially in an acidic environment. This hybrid enzyme has maximum activity between pH 5.6 and 6.6, whereas the pH-optimum for enzymatic activity of B. amyloliquefaciens beta-glucanase was found to be at pH 6 to 7 and for B. macerans at pH 6.0 to 7.5. Hybrid enzyme 1 being more heat stable than both parental enzymes represents a case of intragenic heterosis. Hybrid beta-glucanase 2 (H2) was found to be more thermolabile than the naturally occurring beta-glucanases it was derived from and the pH-optimum for enzymatic activity was determined to be between pH 7 and pH 8.

杂交芽孢杆菌内(1-3,1-4)- β -葡聚糖酶:重组基因的构建及基因产物的分子性质
从解淀粉芽孢杆菌和芽胞杆菌中分离得到的β -葡聚糖酶基因的两半相互交换,构建了杂交基因。β -葡聚糖酶杂交酶1 (H1)含有成熟的解淀粉芽孢杆菌β -葡聚糖酶的107个氨基末端残基和芽孢杆菌β -葡聚糖酶的107个羧基末端氨基酸残基。相互作用的β -葡聚糖酶杂交酶2 (H2)由macerans酶的105个氨基末端残基和解淀粉酵母菌的107个羧基末端残基组成。这两种杂交酶的生化特性彼此之间以及与亲本β -葡聚糖酶之间存在显著差异。与其他β -葡聚糖酶相比,杂交β -葡聚糖酶H1表现出更高的热稳定性,特别是在酸性环境中。该杂交酶在pH 5.6 ~ 6.6之间具有最大活性,而解淀粉芽孢杆菌β -葡聚糖酶的最适酶活pH为6 ~ 7,芽孢杆菌的最适酶活pH为6.0 ~ 7.5。杂种酶1比两个亲本酶都更热稳定,这是种内杂种优势的一个例子。杂交β -葡聚糖酶2 (H2)被发现比天然产生的β -葡聚糖酶更耐热,酶活性的最佳pH值被确定在pH 7和pH 8之间。
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