Processing and secretion of barley (1-3,1-4)-beta-glucanase in yeast.

O Olsen, K K Thomsen
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引用次数: 15

Abstract

DNA segments encoding signal peptides from mouse alpha-amylase, yeast acid phosphatase, and yeast invertase were fused in frame to a barley (1-3,1-4)-beta-glucanase cDNA gene and expressed in yeast cells under the control of the phosphoglycerate kinase gene promoter. Pure beta-glucanase is obtained by gel filtration of concentrated yeast cell supernatant. It was shown that the glucanase pre-protein was specifically processed and the mature protein efficiently secreted when the yeast invertase signal sequence directed secretion.

酵母中大麦(1-3,1-4)- β -葡聚糖酶的加工和分泌。
编码小鼠α -淀粉酶、酵母酸性磷酸酶和酵母转化酶信号肽的DNA片段在框架中融合到大麦(1-3,1-4)- β -葡聚糖酶cDNA基因中,并在磷酸甘油酸激酶基因启动子的控制下在酵母细胞中表达。通过凝胶过滤浓缩酵母细胞上清得到纯葡聚糖酶。结果表明,在酵母转化酶信号序列的指导下,葡聚糖酶前蛋白被特异性加工,成熟蛋白被有效分泌。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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