A closer look at calcium-induced interactions between phosphatidylserine-(PS) doped liposomes and the structural effects caused by inclusion of gangliosides or polyethylene glycol- (PEG) modified lipids

IF 2.8 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Philipp Grad , Katarina Edwards , Lars Gedda , Víctor Agmo Hernández
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Abstract

The effects of polyethylene glycol- (PEG) modified lipids and gangliosides on the Ca2+ induced interaction between liposomes composed of palmitoyl-oleoyl phosphatidylethanolamine (POPE) and palmitoyl-oleoyl phosphatidylserine (POPS) was investigated at physiological ionic strength. Förster resonance energy transfer (FRET) studies complemented with dynamic light scattering (DLS) and cryo-transmission electron microscopy (Cryo-EM) show that naked liposomes tend to adhere, rupture, and collapse on each other's surfaces upon addition of Ca2+, eventually resulting in the formation of large multilamellar aggregates and bilayer sheets. Noteworthy, the presence of gangliosides or PEGylated lipids does not prevent the adhesion-rupture process, but leads to the formation of small, long-lived bilayer fragments/disks. PEGylated lipids seem to be more effective than gangliosides at stabilizing these structures. Attractive interactions arising from ion correlation are proposed to be a driving force for the liposome-liposome adhesion and rupture processes. The results suggest that, in contrast with the conclusions drawn from previous solely FRET-based studies, direct liposome-liposome fusion is not the dominating process triggered by Ca2+ in the systems studied.

Abstract Image

仔细观察钙诱导的磷脂酰丝氨酸(PS)掺杂脂质体之间的相互作用以及包含神经节苷脂或聚乙二醇(PEG)修饰脂质体引起的结构效应。
在生理离子强度下,研究了聚乙二醇(PEG)修饰的脂质和神经节苷对棕榈酰油酰磷脂酰乙醇胺(POPE)和棕榈酰油酰磷脂酰丝氨酸(POPS)组成的脂质体之间Ca2+诱导的相互作用的影响。Förster共振能量转移(FRET)研究与动态光散射(DLS)和冷冻透射电子显微镜(cro - em)相结合表明,在添加Ca2+后,裸脂质体倾向于在彼此的表面粘附,破裂和坍塌,最终导致形成大的多层聚集体和双层片。值得注意的是,神经节苷脂或聚乙二醇化脂的存在并不能阻止粘连破裂过程,而是导致小的、长寿命的双层碎片/磁盘的形成。聚乙二醇化脂类似乎比神经节苷类在稳定这些结构方面更有效。离子相关引起的吸引相互作用被认为是脂质体-脂质体粘附和破裂过程的驱动力。结果表明,与以往仅基于fret的研究得出的结论相反,脂质体与脂质体的直接融合并不是所研究系统中Ca2+触发的主导过程。
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来源期刊
Biochimica et biophysica acta. Biomembranes
Biochimica et biophysica acta. Biomembranes 生物-生化与分子生物学
CiteScore
8.20
自引率
5.90%
发文量
175
审稿时长
2.3 months
期刊介绍: BBA Biomembranes has its main focus on membrane structure, function and biomolecular organization, membrane proteins, receptors, channels and anchors, fluidity and composition, model membranes and liposomes, membrane surface studies and ligand interactions, transport studies, and membrane dynamics.
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