Cis-urocanic acid stereospecifically modulates human monocyte IL-1 production and surface HLA-DR antigen expression, T-cell IL-2 production and CD4/CD8 ratio.

Photo-dermatology Pub Date : 1989-12-01
L Räsänen, C T Jansén, H Hyöty, T Reunala, H Morrison
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Abstract

UV irradiation is known to photoisomerize epidermal trans-urocanic acid (trans-UCA) to cis-urocanic acid (cis-UCA), which has been postulated to be involved in local and systemic downregulation of immune responses. We have earlier shown that cis-UCA suppresses interleukin 1 (IL-1) production in human epidermal cells. To study the possible effects of UCA isomers on human peripheral blood lymphoid cells, these cells were cultured in the presence of either UCA stereoisomer, and a number of immunological parameters were assayed. Cis-UCA (100 micrograms/ml) caused a significant downregulation of monocyte IL-1 production, and diminished monocyte HLA-DR expression. Cis-UCA also caused a significant reduction in the CD4/CD8 ratio. Furthermore, T-cells preincubated with cis-UCA caused a significant downregulation of purified protein derivative-induced interleukin 2 production by autologous T-cells. The trans isomer had no effect in any of these in vitro tests. The reported stereospecific effects of cis-UCA are compatible with the postulated function of this chemical as an UV-induced, low-molecular-weight immunomediator substance.

顺式尿尿酸立体特异性调节人单核细胞IL-1的产生、表面HLA-DR抗原的表达、t细胞IL-2的产生和CD4/CD8比值。
已知紫外线照射可使表皮反式尿醛酸(反式uca)光异构化为顺式尿醛酸(顺式uca),这被认为与局部和全身免疫反应下调有关。我们之前已经表明,顺式uca抑制人表皮细胞中白细胞介素1 (IL-1)的产生。为了研究UCA异构体对人外周血淋巴样细胞可能产生的影响,将这些细胞在UCA立体异构体存在下培养,并测定了一些免疫学参数。Cis-UCA(100微克/毫升)导致单核细胞IL-1产生显著下调,单核细胞HLA-DR表达降低。Cis-UCA还导致CD4/CD8比值显著降低。此外,用顺式uca预孵育的t细胞引起了纯化蛋白衍生物诱导的自体t细胞产生白细胞介素2的显著下调。反式异构体在这些体外试验中没有任何影响。报道的顺式uca的立体特异性效应与该化学物质作为紫外线诱导的低分子量免疫介质的假设功能是相容的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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