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Q1 Agricultural and Biological Sciences

Current protocols in mouse biology Pub Date : 2019-03-05 DOI:10.1002/cpmo.52

引用次数: 0

Abstract

Cover: In Lange et al. (https://doi.org/10.1002/cpmo.59) image shows cecal and colonic fluorescent micrographs from representative GF mice and non-GF control mice housed in an IVC operated in containment mode for 4 weeks. Colon enterocytes were stained with DAPI, and bacterial cells were stained with the bacterial probe EUB338 tagged with an orange fluorescent dye (Alexa 555). (A,B) Cecum (A) and proximal colon (B) from a representative GF mouse showing intestinal digesta within the lumen (asterisks), but no observable bacterial cells. (C,D) Cecum (C) and proximal colon (D) from a representative non-GF control mouse showing abundant bacterial cells within the lumen (arrows). Scale bars, 200 µm.

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发布信息TOC

封面:Lange等人(https://doi.org/10.1002/cpmo.59)的图片显示了代表性的GF小鼠和非GF对照小鼠的盲肠和结肠荧光显微照片，这些小鼠被置于封闭模式下的IVC中4周。结肠肠细胞用DAPI染色，细菌细胞用标记有橙色荧光染料(Alexa 555)的细菌探针EUB338染色。(A,B)代表性GF小鼠的盲肠(A)和近端结肠(B)显示肠内消化(星号)，但未观察到细菌细胞。(C,D)来自代表性非gf对照小鼠的盲肠(C)和近端结肠(D)，显示管腔内丰富的细菌细胞(箭头)。比例尺，200µm。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.