{"title":"A simple and efficient method for isolating genomic DNA from endomycorrhizal spores","authors":"Brian Cummings, Tim Wood","doi":"10.1016/0735-0651(89)90013-7","DOIUrl":null,"url":null,"abstract":"<div><p>A procedure for rapid isolation of genomic DNA from spores of endomycorrhizal fungi is described. Isolation of high molecular weight DNA relies on the lysis of freeze-dried spores and extraction of DNA using mixed alkyl trimethyl ammonium bromide and an excess of proteinase. DNA greater than 30 kb was successfully isolated from <em>Glomus mosseae, Glomus intraradices</em>, and <em>Glomus etnuicatum</em> using less than 600,000 spores. All DNA preparations were suitable for restriction analysis, hybridizations, and cloning.</p></div>","PeriodicalId":77714,"journal":{"name":"Gene analysis techniques","volume":"6 5","pages":"Pages 89-92"},"PeriodicalIF":0.0000,"publicationDate":"1989-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0735-0651(89)90013-7","citationCount":"13","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gene analysis techniques","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0735065189900137","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 13
Abstract
A procedure for rapid isolation of genomic DNA from spores of endomycorrhizal fungi is described. Isolation of high molecular weight DNA relies on the lysis of freeze-dried spores and extraction of DNA using mixed alkyl trimethyl ammonium bromide and an excess of proteinase. DNA greater than 30 kb was successfully isolated from Glomus mosseae, Glomus intraradices, and Glomus etnuicatum using less than 600,000 spores. All DNA preparations were suitable for restriction analysis, hybridizations, and cloning.
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